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Using NMR spectra and mass spectrometry analyses of the extract we identified eight main constituents: oleic acid (\n                    <jats:bold>1<\/jats:bold>\n                    ), octadecenoic acid (\n                    <jats:bold>2<\/jats:bold>\n                    ), stigmasterol (\n                    <jats:bold>3<\/jats:bold>\n                    ),\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -amyrin (\n                    <jats:bold>4<\/jats:bold>\n                    ), pinoresinol (\n                    <jats:bold>5<\/jats:bold>\n                    ), 6 methoxypodophyllotoxin (\n                    <jats:bold>6<\/jats:bold>\n                    ), coniferin (\n                    <jats:bold>7<\/jats:bold>\n                    ), and 6-methoxypodophyllotoxin-7-\n                    <jats:italic>O<\/jats:italic>\n                    -\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -D-glucopyranoside (\n                    <jats:bold>8<\/jats:bold>\n                    ). By using the sulforhodamine B assay, an important cytotoxic activity against four human cancer cell lines, HF6 colon (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 0.57\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL), MCF7 breast (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 0.56\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL), PC3 prostate (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.60\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL), and SiHa cervical (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.54\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL), as well as toward the normal fibroblasts line HFS-30 IC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.02\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL was demonstrated. Compound\n                    <jats:bold>6<\/jats:bold>\n                    (6-methoxypodophyllotoxin) was responsible for the cytotoxic activity exhibiting an IC\n                    <jats:sub>50<\/jats:sub>\n                    value range of 0.0632 to 2.7433\u2009\n                    <jats:italic>\u00b5<\/jats:italic>\n                    g\/mL against the tested cell lines. Cell cycle studies with compound\n                    <jats:bold>6<\/jats:bold>\n                    exhibited a cell arrest in G2\/M of the prostate PC3 cancer cell line. Microtubule disruption studies demonstrated that compound\n                    <jats:bold>6<\/jats:bold>\n                    inhibited the polymerization of tubulin through its binding to the colchicine site (binding constant\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>K<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mi>b<\/mml:mi>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                      <mml:mo>=<\/mml:mo>\n                      <mml:mn>7.6<\/mml:mn>\n                      <mml:mo>\u00d7<\/mml:mo>\n                      <mml:msup>\n                        <mml:mrow>\n                          <mml:mn>10<\/mml:mn>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mn>6<\/mml:mn>\n                        <\/mml:mrow>\n                      <\/mml:msup>\n                      <mml:mo>\u2009<\/mml:mo>\n                      <mml:msup>\n                        <mml:mrow>\n                          <mml:mtext>M<\/mml:mtext>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mo>-<\/mml:mo>\n                          <mml:mn>1<\/mml:mn>\n                        <\/mml:mrow>\n                      <\/mml:msup>\n                    <\/mml:math>\n                    ). A dose-response apoptotic effect was also observed. This work constitutes the first investigation reporting the chemical composition of\n                    <jats:italic>L. scabrellum<\/jats:italic>\n                    and the first study determining the mechanism of action of compound\n                    <jats:bold>6<\/jats:bold>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/2015\/298463","type":"journal-article","created":{"date-parts":[[2015,7,12]],"date-time":"2015-07-12T17:00:53Z","timestamp":1436720453000},"page":"1-11","source":"Crossref","is-referenced-by-count":11,"title":["Cytotoxic Activity and Chemical Composition of the Root Extract from the Mexican Species\n                    <i>Linum scabrellum<\/i>\n                    : Mechanism of Action of the Active Compound 6-Methoxypodophyllotoxin"],"prefix":"10.1155","volume":"2015","author":[{"given":"Ivonne","family":"Alejandre-Garc\u00eda","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Cardoso-Taketa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Gonz\u00e1lez-Maya","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra","family":"Ant\u00fanez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Salas-Vidal","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 62209 Cuernavaca, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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