{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:28:46Z","timestamp":1791217726288,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Escherichia coli<\/jats:italic>remains as one of the most important bacteria causing infections in pediatrics and producing extended-spectrum beta-lactamases (ESBLs) making them resistant to beta-lactam antibiotics. In this study we aimed to genotype ESBL-producing<jats:italic>E. coli<\/jats:italic>isolates from pediatric patients for ESBL genes and determine their association with antimicrobial resistance. One hundred of the<jats:italic>E. coli<\/jats:italic>isolates were initially considered ESBL producing based on their MIC results. These isolates were then tested by polymerase chain reaction (PCR) for the presence or absence of<jats:italic>CTX<\/jats:italic>,<jats:italic>TEM<\/jats:italic>,<jats:italic>SHV<\/jats:italic>,<jats:italic>GES<\/jats:italic>, and<jats:italic>VEB<\/jats:italic>beta-lactamase genes. About 30.5% of isolated<jats:italic>E. coli<\/jats:italic>was ESBL-producing strain. The<jats:italic>TEM<\/jats:italic>gene was the most prevalent (49%) followed by<jats:italic>SHV<\/jats:italic>(44%),<jats:italic>CTX<\/jats:italic>(28%),<jats:italic>VEB<\/jats:italic>(8%), and<jats:italic>GES<\/jats:italic>(0%) genes. The ESBL-producing<jats:italic>E. coli<\/jats:italic>isolates were susceptible to carbapenems (66%) and amikacin (58%) and showed high resistance to cefixime (99%), colistin (82%), and ciprofloxacin (76%). In conclusion, carbapenems were the most effective antibiotics against ESBl-producing<jats:italic>E. coli<\/jats:italic>in urinary tract infection in North of Iran. The most prevalent gene is the TEM-type, but the other resistant genes and their antimicrobial resistance are on the rise.<\/jats:p>","DOI":"10.1155\/2015\/309478","type":"journal-article","created":{"date-parts":[[2015,5,3]],"date-time":"2015-05-03T21:03:35Z","timestamp":1430687015000},"page":"1-7","source":"Crossref","is-referenced-by-count":54,"title":["Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-Producing<i>Escherichia coli<\/i>among Uropathogens of Pediatrics in North of Iran"],"prefix":"10.1155","volume":"2015","author":[{"given":"Mohammad Sadegh","family":"Rezai","sequence":"first","affiliation":[{"name":"Nosocomial Infection Research Center, Mazandaran University of Medical Sciences, Buali Sina Hospital, Sari, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim","family":"Salehifar","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy, Faculty of Pharmacy, Thalassemia Research Center, Mazandaran 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