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Human umbilical cord mesenchymal stem cells (UC-MSCs) can regulate the function of immune cells. However, whether and how UC-MSCs can modulate the function of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells has not been fully understood. <jats:italic>Methods<\/jats:italic>. The PBMCs or V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells were activated and expanded with pamidronate (PAM) and interleukin-2 (IL-2) with or without the presence UC-MSCs. The effects of UC-MSCs on the proliferation, cytokine expression, and cytotoxicity of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells were determined by flow cytometry. The effects of UC-MSCs on Fas-L, TRAIL-expressing V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells, and V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cell apoptosis were determined by flow cytometry. <jats:italic>Results<\/jats:italic>. UC-MSCs inhibited V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cell proliferation in a dose-dependent but cell-contact independent manner. Coculture with UC-MSCs reduced the frequency of IFN<jats:italic>\u03b3<\/jats:italic>+ but increased granzyme B+ V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells. UC-MSCs inhibited the cytotoxicity of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells against influenza virus H1N1 infected A549 cells and also reduced the frequency of Fas-L+, TRAIL+ V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells but failed to modulate the apoptosis of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells. <jats:italic>Conclusions<\/jats:italic>. These results indicated that UC-MSCs efficiently suppressed the proliferation and cytotoxicity of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells and modulated their cytokine production. Fas-L and TRAIL were involved in the regulation. Cell contact and apoptosis of V<jats:italic>\u03b3<\/jats:italic>9V<jats:italic>\u03b4<\/jats:italic>2 T cells were not necessary for the inhibition.<\/jats:p>","DOI":"10.1155\/2015\/317801","type":"journal-article","created":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T17:02:37Z","timestamp":1429030957000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated V<i>\u03b3<\/i>9V<i>\u03b4<\/i>2 T Lymphocytes In Vitro"],"prefix":"10.1155","volume":"2015","author":[{"given":"Xiaohuan","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Obstetrics & Gynecology and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, China"},{"name":"Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, 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