{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:16:12Z","timestamp":1790172972910,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T00:00:00Z","timestamp":1445990400000},"content-version":"vor","delay-in-days":300,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Seoul National University Dental Hospital"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> is considered with inducing pulpal inflammation and has lipopolysaccharide (LPS) as an inflammatory stimulator. GV1001 peptide has anticancer and anti\u2010inflammation activity due to inhibiting activation of signaling molecules after penetration into the various types of cells. Therefore, this study examined inhibitory effect of GV1001 on dental pulp cells (hDPCs) stimulated by <jats:italic>P. gingivalis<\/jats:italic> LPS. The intracellular distribution of GV1001 was analyzed by confocal microscopy. Real\u2010time RT\u2010PCR was performed to determine the expression levels of TNF\u2010<jats:italic>\u03b1<\/jats:italic> and IL\u20106 cytokines. The role of signaling by MAP kinases (ERK and p38) was explored using Western blot analysis. The effect of GV1001 peptide on hDPCs viability was measured by MTT assay.  GV1001 was predominantly located in hDPC cytoplasm. The peptide inhibited <jats:italic>P. gingivalis<\/jats:italic> LPS\u2010induced TNF\u2010<jats:italic>\u03b1<\/jats:italic> and IL\u20106 production in hDPCs without significant cytotoxicity. Furthermore, GV1001 treatment markedly inhibited the phosphorylation of MAP kinases (ERK and p38) in LPS\u2010stimulated hDPCs. GV1001 may prevent <jats:italic>P. gingivalis<\/jats:italic> LPS\u2010induced inflammation of apical tissue. Also, these findings provide mechanistic insight into how GV1001 peptide causes anti\u2010inflammatory actions in LPS\u2010stimulated pulpitis without significantly affecting cell viability.<\/jats:p>","DOI":"10.1155\/2015\/385127","type":"journal-article","created":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T23:00:35Z","timestamp":1446073235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["The Anti\u2010Inflammatory Effect of Human Telomerase\u2010Derived Peptide on <i>P. gingivalis<\/i> Lipopolysaccharide\u2010Induced Inflammatory Cytokine Production and Its Mechanism in Human Dental Pulp Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"Yoo-Jin","family":"Ko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kil-Young","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee-Yeon","family":"Kum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo-Cheol","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Ho","family":"Baek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo K.","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Jun","family":"Shon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,28]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.07.001"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508401105"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508401105"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072682"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.9.7601337"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.68.2.320-344.2004"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20967"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2012.02096.x"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)00210-5"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00492-5"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90170-9"},{"key":"e_1_2_8_12_2","first-page":"S68","article-title":"Telomerase activity in human solid tumors. 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