{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T18:46:52Z","timestamp":1774118812811,"version":"3.50.1"},"reference-count":22,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Following fetal or neonatal gene transfer in mice and other species immune tolerance of the transgenic protein is frequently observed; however the underlying mechanisms remain largely undefined. In this study fetal and neonatal BALB\/c mice received adenovirus vector to deliver human factor IX (hFIX) cDNA. The long-term tolerance of hFIX was robust in the face of immune challenge with hFIX protein and adjuvant but was eliminated by simultaneous administration of anti-CD25+ antibody. Naive irradiated BALB\/c mice which had received lymphocytes from donors immunised with hFIX developed anti-hFIX antibodies upon immune challenge. Cotransplantation with CD4+CD25+ cells isolated from neonatally tolerized donors decreased the antibody response. In contrast, cotransplantation with CD4+CD25\u2212 cells isolated from the same donors increased the antibody response. These data provide evidence that immune tolerance following perinatal gene transfer is maintained by a CD4+CD25+ regulatory population.<\/jats:p>","DOI":"10.1155\/2015\/397879","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T21:00:30Z","timestamp":1422738030000},"page":"1-6","source":"Crossref","is-referenced-by-count":12,"title":["Evidence for Contribution of CD4+CD25+ Regulatory T Cells in Maintaining Immune Tolerance to Human Factor IX following Perinatal Adenovirus Vector Delivery"],"prefix":"10.1155","volume":"2015","author":[{"given":"Megha S.","family":"Nivsarkar","sequence":"first","affiliation":[{"name":"Gene Transfer Technology Group, University College London, 86-96 Chenies Mews, London WC1E 6HXZ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne M. 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