{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:38:41Z","timestamp":1787585921039,"version":"build-2736575974"},"reference-count":42,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Ministry of Science, Education and Sports of the Republic of Croatia","award":["006-0061117-1238"],"award-info":[{"award-number":["006-0061117-1238"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>The present study aimed to evaluate antioxidant and acetylcholinesterase (AChE) inhibitory activities of the ethanolic extracts of six selected<jats:italic>Thymus<\/jats:italic>species growing in Croatia (<jats:italic>T. longicaulis<\/jats:italic>,<jats:italic>T. praecox<\/jats:italic>subsp.<jats:italic>polytrichus<\/jats:italic>,<jats:italic>T. pulegioides<\/jats:italic>,<jats:italic>T. serpyllum<\/jats:italic>subsp.<jats:italic>serpyllum<\/jats:italic>,<jats:italic>T. striatus<\/jats:italic>, and<jats:italic>T. vulgaris<\/jats:italic>). Antioxidant effectiveness was assessed using six different assays, in comparison with rosmarinic acid, luteolin, and reference antioxidants. All tested<jats:italic>Thymus<\/jats:italic>extracts possessed DPPH (IC<jats:sub>50<\/jats:sub>= 3\u20136\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) and nitric oxide (IC<jats:sub>50<\/jats:sub>= 70\u2013177\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) free radical scavenging activities, strong reducing properties (IC<jats:sub>50<\/jats:sub>= 11\u201315\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), ferrous ion chelating activity (IC<jats:sub>50<\/jats:sub>= 126\u2013389\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), ability to inhibit lipid peroxidation (IC<jats:sub>50<\/jats:sub>= 34\u201380\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), and high total antioxidant capacities (238\u2013294\u2009mg\u2009AAE\/g). AChE inhibitory activity was examined using Ellman's colorimetric method and all tested extracts showed anti-AChE activity in a dose dependent manner. The values of 10\u201328%, 23\u201339%, and 64\u201386% were obtained for tested concentrations of 0.25, 0.5, and 1\u2009mg\/mL, respectively. Additionally, the contents of total hydroxycinnamic derivatives, flavonoids, and tannins in dried plant samples were determined spectrophotometrically. Our results highlighted<jats:italic>Thymus<\/jats:italic>species as a rich source of natural antioxidants and AChE inhibitors that could be useful in preventing and treating Alzheimer\u2019s disease and other neurodegenerative disorders.<\/jats:p>","DOI":"10.1155\/2015\/403950","type":"journal-article","created":{"date-parts":[[2015,8,16]],"date-time":"2015-08-16T17:03:06Z","timestamp":1439744586000},"page":"1-10","source":"Crossref","is-referenced-by-count":54,"title":["Antioxidant and Anticholinesterase Potential of Six<i>Thymus<\/i>Species"],"prefix":"10.1155","volume":"2015","author":[{"given":"Marija","family":"Kindl","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Maruli\u0107ev trg 20, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biljana","family":"Bla\u017eekovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty 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