{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:49:24Z","timestamp":1790502564475,"version":"4.1.0"},"reference-count":20,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Qianjiang Talents C and D Scheme of Zhejiang Province","award":["QJD1302020"],"award-info":[{"award-number":["QJD1302020"]}]},{"name":"Qianjiang Talents C and D Scheme of Zhejiang Province","award":["2011R09042-07"],"award-info":[{"award-number":["2011R09042-07"]}]},{"name":"Zhejiang Province Key Science and Technology Innovation Program","award":["QJD1302020"],"award-info":[{"award-number":["QJD1302020"]}]},{"name":"Zhejiang Province Key Science and Technology Innovation Program","award":["2011R09042-07"],"award-info":[{"award-number":["2011R09042-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Two peptides of Pt-A (Glu-Asn-Trp 429\u2009Da) and Pt-B (Glu-Gln-Trp 443\u2009Da) were isolated from venom liquor of<jats:italic>Deinagkistrodon acutus<\/jats:italic>. Their antiplatelet aggregation effects were evaluated with platelet-rich human plasma<jats:italic>in vitro<\/jats:italic>; the respective IC<jats:sub>50<\/jats:sub>of Pt-A and Pt-B was 66\u2009<jats:italic>\u03bc<\/jats:italic>M and 203\u2009<jats:italic>\u03bc<\/jats:italic>M. Both peptides exhibited protection effects on ADP-induced paralysis in mice. After ADP administration, the paralysis time of different concentration of Pt-A and Pt-B lasted as the following: 80\u2009mg\/kg Pt-B (152.8 \u00b1 57.8\u2009s) &lt; 40\u2009mg\/kg Pt-A (163.5 \u00b1 59.8\u2009s) &lt; 20\u2009mg\/kg Pt-A (253.5 \u00b1 74.5\u2009s) &lt; 4\u2009mg\/kg clopidogrel (a positive control, 254.5 \u00b1 41.97\u2009s) &lt; 40\u2009mg\/kg Pt-B (400.8 \u00b1 35.9\u2009s) &lt; 10\u2009mg\/kg Pt-A (422.8 \u00b1 55.4\u2009s), all of which were statistically shorter than the saline treatment (666 \u00b1 28\u2009s). Pulmonary tissue biopsy confirmed that Pt-A and Pt-B prevented the formation of thrombi in the lung. Unlike ADP injection alone, which caused significant reduction of peripheral platelet count, Pt-A treatment prevented the drop of peripheral platelet counts; interestingly, Pt-B could not, even though the same amount of Pt-B also showed protection effects on ADP-induced paralysis and thrombosis. More importantly, intravenous injection of Pt-A and Pt-B did not significantly increase the hemorrhage risks as clopidogrel.<\/jats:p>","DOI":"10.1155\/2015\/412841","type":"journal-article","created":{"date-parts":[[2015,9,21]],"date-time":"2015-09-21T21:02:23Z","timestamp":1442869343000},"page":"1-6","source":"Crossref","is-referenced-by-count":12,"title":["Antiplatelet Aggregation and Antithrombosis Efficiency of Peptides in the Snake Venom of<i>Deinagkistrodon acutus<\/i>: Isolation, Identification, and Evaluation"],"prefix":"10.1155","volume":"2015","author":[{"given":"Bin","family":"Ding","sequence":"first","affiliation":[{"name":"Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghong","family":"Xu","sequence":"additional","affiliation":[{"name":"Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, 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