{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:41:15Z","timestamp":1790394075123,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. To study the potential of long interspersed element-1 (LINE-1) methylation change in the prediction of postmolar gestational trophoblastic neoplasia (GTN).<jats:italic>Methods<\/jats:italic>. The LINE-1 methylation pattern from first trimester placenta, hydatidiform mole, and malignant trophoblast specimens were compared. Then, hydatidiform mole patients from 11999 to 2010 were classified into the following 2 groups: a remission group and a group that developed postmolar GTN. Specimens were prepared for a methylation study. The methylation levels and percentages of LINE-1 loci were evaluated for their sensitivity, specificity, and accuracy for the prediction of postmolar GTN.<jats:italic>Results<\/jats:italic>. First, 12 placentas, 38 moles, and 19 malignant trophoblast specimens were compared. The hydatidiform mole group had the highest LINE-1 methylation level (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>p<\/mml:mi><\/mml:mrow><\/mml:math>= 0.003) and the<jats:sup>u<\/jats:sup>C<jats:sup>u<\/jats:sup>C of LINE-1 increased in the malignant trophoblast group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>p<\/mml:mi><\/mml:mrow><\/mml:math>\u2264 0.001). One hundred forty-five hydatidiform mole patients were classified as 103 remission and 42 postmolar GTN patients. The %<jats:sup>m<\/jats:sup>C<jats:sup>u<\/jats:sup>C and %<jats:sup>u<\/jats:sup>C<jats:sup>m<\/jats:sup>C of LINE-1 showed the lowest<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>p<\/mml:mi><\/mml:mrow><\/mml:math>value for distinguishing between the two groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:mi>p<\/mml:mi><\/mml:mrow><\/mml:math>&lt; 0.001). The combination of the pretreatment<jats:italic>\u03b2<\/jats:italic>-hCG level (\u2265100,000\u2009mIU\/mL) with the %<jats:sup>m<\/jats:sup>C<jats:sup>u<\/jats:sup>C and %<jats:sup>u<\/jats:sup>C<jats:sup>m<\/jats:sup>C, sensitivity, specificity, PPV, NPV, and accuracy modified the levels to 60.0%, 92.2%, 77.4%, 83.8%, and 82.3%, respectively.<jats:italic>Conclusions<\/jats:italic>. A reduction in the partial methylation of LINE-1 occurs early before the clinical appearance of malignant transformation. The %<jats:sup>m<\/jats:sup>C<jats:sup>u<\/jats:sup>C and %<jats:sup>u<\/jats:sup>C<jats:sup>m<\/jats:sup>C of LINE-1s may be promising markers for monitoring hydatidiform moles before progression to GTN.<\/jats:p>","DOI":"10.1155\/2015\/421747","type":"journal-article","created":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T02:18:18Z","timestamp":1442197098000},"page":"1-7","source":"Crossref","is-referenced-by-count":13,"title":["LINE-1 Methylation Patterns as a Predictor of Postmolar Gestational Trophoblastic Neoplasia"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3516-0584","authenticated-orcid":true,"given":"Ruangsak","family":"Lertkhachonsuk","sequence":"first","affiliation":[{"name":"Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krissada","family":"Paiwattananupant","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patou","family":"Tantbirojn","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Pathology, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakasit","family":"Rattanatanyong","sequence":"additional","affiliation":[{"name":"Center of Excellence in Molecular Genetic of Cancer and Human Disease, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, 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