{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:04:49Z","timestamp":1789679089448,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["R01 HL105826"],"award-info":[{"award-number":["R01 HL105826"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["K02 HL114749"],"award-info":[{"award-number":["K02 HL114749"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["5RO1 HL081734-09"],"award-info":[{"award-number":["5RO1 HL081734-09"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["15PRE22430028"],"award-info":[{"award-number":["15PRE22430028"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["14GRNT20490025"],"award-info":[{"award-number":["14GRNT20490025"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Cardiomyopathies can result from mutations in genes encoding sarcomere proteins including<jats:italic>MYBPC3<\/jats:italic>, which encodes cardiac myosin binding protein-C (cMyBP-C). However, whether oxidative stress is augmented due to contractile dysfunction and cardiomyocyte damage in<jats:italic>MYBPC3<\/jats:italic>-mutated cardiomyopathies has not been elucidated. To determine whether oxidative stress markers were elevated in<jats:italic>MYBPC3<\/jats:italic>-mutated cardiomyopathies, a previously characterized 3-month-old mouse model of dilated cardiomyopathy (DCM) expressing a homozygous<jats:italic>MYBPC3<\/jats:italic>mutation (cMyBP-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant=\"normal\">C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi mathvariant=\"normal\">t<\/mml:mi><mml:mo>\/<\/mml:mo><mml:mi mathvariant=\"normal\">t<\/mml:mi><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>) was used, compared to wild-type (WT) mice. Echocardiography confirmed decreased percentage of fractional shortening in DCM versus WT hearts. Histopathological analysis indicated a significant increase in myocardial disarray and fibrosis while the second harmonic generation imaging revealed disorganized sarcomeric structure and myocyte damage in DCM hearts when compared to WT hearts. Intriguingly, DCM mouse heart homogenates had decreased glutathione (GSH\/GSSG) ratio and increased protein carbonyl and lipid malondialdehyde content compared to WT heart homogenates, consistent with elevated oxidative stress. Importantly, a similar result was observed in human cardiomyopathy heart homogenate samples. These results were further supported by reduced signals for mitochondrial semiquinone radicals and Fe-S clusters in DCM mouse hearts measured using electron paramagnetic resonance spectroscopy. In conclusion, we demonstrate elevated oxidative stress in<jats:italic>MYPBC3<\/jats:italic>-mutated DCM mice, which may exacerbate the development of heart failure.<\/jats:p>","DOI":"10.1155\/2015\/424751","type":"journal-article","created":{"date-parts":[[2015,10,5]],"date-time":"2015-10-05T21:03:31Z","timestamp":1444079011000},"page":"1-14","source":"Crossref","is-referenced-by-count":36,"title":["Oxidative Stress in Dilated Cardiomyopathy Caused by<i>MYBPC3<\/i>Mutation"],"prefix":"10.1155","volume":"2015","author":[{"given":"Thomas L.","family":"Lynch","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Physiology, Health Sciences Division, Loyola University Chicago, Maywood, IL 60153, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayandi","family":"Sivaguru","sequence":"additional","affiliation":[{"name":"Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murugesan","family":"Velayutham","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo J.","family":"Cardounel","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Michels","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Thoraxcenter, Erasmus Medical Center, \u2019s-Gravendijkwal 230, 3015 CE Rotterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Barefield","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Physiology, Health Sciences Division, Loyola University Chicago, Maywood, IL 60153, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Govindan","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Physiology, Health Sciences Division, Loyola University Chicago, Maywood, IL 60153, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristobal dos","family":"Remedios","sequence":"additional","affiliation":[{"name":"Bosch Institute, Discipline of Anatomy and Histology, University of Sydney, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanda","family":"van der Velden","sequence":"additional","affiliation":[{"name":"Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakthivel","family":"Sadayappan","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Physiology, Health Sciences Division, Loyola University Chicago, Maywood, IL 60153, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000441139.02102.80"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm084"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60397-3"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.108.838672"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-011-9268-3"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62862"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn078"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.10.041"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.108.790501"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.112.191478"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7377"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00913.2013"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.4.383"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000126898.95550.31"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-756-3_18"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2012.931"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-8981(03)00003-2"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1179\/135100009x392601"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(91)90192-6"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00508.2010"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr087"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2010.540242"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.071555"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1364\/oe.18.024983"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.07.016"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(09)70091-X"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-9877(96)90453-3"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.3109\/10715769609145656"},{"key":"30","doi-asserted-by":"crossref","first-page":"715S","DOI":"10.1093\/ajcn\/57.5.715S","volume":"57","year":"1993","journal-title":"The American Journal of Clinical Nutrition"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.04.016"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1172\/jci7377"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1117\/1.2967536"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.05.065"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.000374"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.010908.163111"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.06.058"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00388-0"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.2007.s96"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00554.2011"},{"issue":"1","key":"41","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1093\/clinchem\/44.1.148","volume":"44","year":"1998","journal-title":"Clinical Chemistry"},{"issue":"2","key":"42","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0026-0495(00)80077-3","volume":"49","year":"2000","journal-title":"Metabolism"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(01)00724-9"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm005"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(96)00327-9"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.2.152"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.4.357"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.01.003"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m005804200"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2015\/424751.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2015\/424751.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2015\/424751.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T06:17:52Z","timestamp":1599632272000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/omcl\/2015\/424751\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":49,"alternative-id":["424751","424751"],"URL":"https:\/\/doi.org\/10.1155\/2015\/424751","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}