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We investigated if miR\u2010208a has proapoptotic effects on ischemic cardiomyocytes and if its silencing has therapeutic benefits in MI. <jats:italic>Methods and Results<\/jats:italic>. The effect of miR\u2010208a on apoptosis during ischemia was studied in cultured neonatal mice myocytes transfected with agomir or antagomir. Differential gene expression was assessed using microarrays. MI was induced in male C57BL\/6 mice randomly assigned to antagomir (<jats:italic>n<\/jats:italic> = 6) or control group (<jats:italic>n<\/jats:italic> = 7), while sham group (<jats:italic>n<\/jats:italic> = 7) had sham operation done. Antagomir group received miR208a antagomir, while control and sham group mice received vehicle only. At 7 and 28 days, echocardiography was done and thereafter hearts were harvested for analysis of apoptosis by TUNEL method, fibrosis using Masson\u2019s trichrome, and hypertrophy using hematoxylin and eosin. miR\u2010208a altered apoptosis genes expression and increased apoptosis in ischemic cardiomyocytes. Therapeutic inhibition of miR\u2010208a decreased cardiac fibrosis, hypertrophy, and apoptosis and significantly improved cardiac function 28 days after MI. <jats:italic>Conclusion<\/jats:italic>. miR\u2010208a alters apoptosis genes expression and promotes apoptosis in ischemic cardiomyocytes, and its silencing attenuates apoptosis, fibrosis, and hypertrophy after MI, with significant improvement in cardiac function.<\/jats:p>","DOI":"10.1155\/2015\/479123","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T21:01:58Z","timestamp":1448485318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["MicroRNA\u2010208a Dysregulates Apoptosis Genes Expression and Promotes Cardiomyocyte Apoptosis during Ischemia and Its Silencing Improves Cardiac Function after Myocardial Infarction"],"prefix":"10.1155","volume":"2015","author":[{"given":"Hasahya","family":"Tony","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bangwei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aijia","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiutang","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunwu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yucheng","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,25]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.6.H2726"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci32490"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00053.2004"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920210793175895"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci36154"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.111.030932"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.10.013"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139089"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000268088"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2010.01.002"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.12.007"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401833101"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/fjc.0b013e3182592d7b"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000148632.35500.d9"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101547"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddmec.2007.12.006"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.101.25.2981"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2012.01523.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e328348ef8e"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214546268"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.110.964684"},{"key":"e_1_2_7_25_2","first-page":"639","article-title":"The role of the cyclin-dependent kinase inhibitor p21 in apoptosis","volume":"1","author":"Gartel A. 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