{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:17:01Z","timestamp":1790713021577,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2011HL013"],"award-info":[{"award-number":["ZR2011HL013"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["2014QY16"],"award-info":[{"award-number":["2014QY16"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Foundation of Shihezi","award":["ZR2011HL013"],"award-info":[{"award-number":["ZR2011HL013"]}]},{"name":"Science and Technology Foundation of Shihezi","award":["2014QY16"],"award-info":[{"award-number":["2014QY16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia\/reperfusion (I\/R) injury in rats.<jats:italic>Method<\/jats:italic>. Left ventricular developed pressure (LVDP) and its maximum up\/down rate (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mo>\u00b1<\/mml:mo><mml:mi>d<\/mml:mi><mml:mi>p<\/mml:mi><mml:mo>\/<\/mml:mo><mml:msub><mml:mrow><mml:mi>d<\/mml:mi><mml:mi>t<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>max<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:math>) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione\/glutathione disulfide (GSH\/GSSG) ratio, and tumor necrosis factor-alpha (TNF-<jats:italic>\u03b1<\/jats:italic>) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3<jats:italic>\u03b2<\/jats:italic>(GSK-3<jats:italic>\u03b2<\/jats:italic>), phospho-GSK-3<jats:italic>\u03b2<\/jats:italic>(P-GSK-3<jats:italic>\u03b2<\/jats:italic>), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.<jats:italic>Results<\/jats:italic>. Pretreatment with kaempferol significantly improved the recovery of LVDP and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mo>\u00b1<\/mml:mo><mml:mi>d<\/mml:mi><mml:mi>p<\/mml:mi><mml:mo>\/<\/mml:mo><mml:msub><mml:mrow><mml:mi>d<\/mml:mi><mml:mi>t<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>max<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:math>, as well as increased the levels of SOD and P-GSK-3<jats:italic>\u03b2<\/jats:italic>and GSH\/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-<jats:italic>\u03b1<\/jats:italic>.<jats:italic>Conclusion<\/jats:italic>. These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3<jats:italic>\u03b2<\/jats:italic>activity in rats with I\/R.<\/jats:p>","DOI":"10.1155\/2015\/481405","type":"journal-article","created":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T17:03:00Z","timestamp":1437584580000},"page":"1-8","source":"Crossref","is-referenced-by-count":106,"title":["Protective Effects of Kaempferol against Myocardial Ischemia\/Reperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi mathvariant=\"italic\">\u03b2<\/mml:mi><\/mml:mrow><\/mml:math>"],"prefix":"10.1155","volume":"2015","author":[{"given":"Mingjie","family":"Zhou","sequence":"first","affiliation":[{"name":"Shandong Provincial Qianfoshan Hospital, Jinan 250014, China"},{"name":"Weifang Medical University, Weifang 261031, 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