{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:06:06Z","timestamp":1792181166197,"version":"4.4.0"},"reference-count":17,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Previously, we reported that a compound from a group of thiohydrazides of oxamic acids, CL-55, possessed antichlamydial activity<jats:italic>in vitro<\/jats:italic>that was accompanied by a decreased translocation of the type three secretion effector, IncA, into the host cell. In this study, the antichlamydial activity of CL-55 was tested<jats:italic>in vivo<\/jats:italic>in DBA\/2 mice infected with<jats:italic>C. trachomatis<\/jats:italic>serovar D. We found that intravaginal inoculation of DBA\/2 mice with the clinically relevant strain,<jats:italic>C. trachomatis<\/jats:italic>serovar D, results in a course of infection and pathology similar to that observed in humans. The early stage of infection in this model was characterized by a shedding of<jats:italic>Chlamydia<\/jats:italic>in vaginal secretions followed by an ascending infection and inflammation in the upper genital tract. We found that CL-55 possessed antibacterial activity<jats:italic>in vivo<\/jats:italic>and was able to control<jats:italic>C. trachomatis<\/jats:italic>vaginal shedding, ascending infection, and inflammation in the upper genital organs in DBA\/2 mice. Our data provide a proof of concept for the protective effect of the thiadiazinon, CL-55, against chlamydial infection<jats:italic>in vivo<\/jats:italic>and support the feasibility of further studies of its potential therapeutic applications.<\/jats:p>","DOI":"10.1155\/2015\/484853","type":"journal-article","created":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T21:02:08Z","timestamp":1422478928000},"page":"1-7","source":"Crossref","is-referenced-by-count":27,"title":["Small Molecule Inhibitor of Type Three Secretion Suppresses Acute and Chronic<i>Chlamydia trachomatis<\/i>Infection in a Novel Urogenital Chlamydia Model"],"prefix":"10.1155","volume":"2015","author":[{"given":"Ekaterina A.","family":"Koroleva","sequence":"first","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia V.","family":"Kobets","sequence":"additional","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor S.","family":"Zayakin","sequence":"additional","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei I.","family":"Luyksaar","sequence":"additional","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila A.","family":"Shabalina","sequence":"additional","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naylia A.","family":"Zigangirova","sequence":"additional","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Russian Federation, Gamaleya Street 18, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"98","reference":[{"key":"1","year":"2012"},{"key":"2","year":"2007"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.8.672"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/art.27394"},{"issue":"2","key":"5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.32607\/20758251-2012-4-2-87-97","volume":"4","year":"2012","journal-title":"Acta Naturae"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir552"},{"issue":"4","key":"7","first-page":"605","volume":"67","year":"1986","journal-title":"British Journal of Experimental Pathology"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0880295"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1128\/aac.35.3.430"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1097(81)90004-5"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1128\/aac.38.9.1937"},{"issue":"1","key":"12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1128\/IAI.2.1.24-28.1970","volume":"2","year":"1970","journal-title":"Infection and Immunity"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.9.6237"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/002261-0"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099521"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.11.100"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2012.43"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/484853.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/484853.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/484853.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T12:13:19Z","timestamp":1598703199000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/484853\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":17,"alternative-id":["484853","484853"],"URL":"https:\/\/doi.org\/10.1155\/2015\/484853","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}