{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:28:35Z","timestamp":1740169715089,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003810","name":"Hospital de Cl\u00ednicas de Porto Alegre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003810","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>RC-3095, a selective GRPR antagonist, has been shown to have anti-inflammatory properties in different models of inflammation. However, its protective effect on lungs submitted to lung ischemia-reperfusion injury has not been addressed before. Then, we administrated RC-3095 intravenously before and after lung reperfusion using an animal model of lung ischemia-reperfusion injury (LIRI) by clamping the pulmonary hilum. Twenty Wistar rats were subjected to an experimental model in four groups: SHAM, ischemia-reperfusion (IR), RC-Pre, and RC-Post. The final mean arterial pressure significantly decreased in IR and RC-Pre compared to their values before reperfusion (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>). The RC-Post group showed significant decrease of partial pressure of arterial oxygen at the end of the observation when compared to baseline (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.005<\/mml:mn><\/mml:math>). Caspase-9 activity was significantly higher in the RC-Post as compared to the other groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.013<\/mml:mn><\/mml:math>). No significant differences were observed in eNOS activity among the groups. The groups RC-Pre and RC-Post did not show any significant decrease in IL-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn>1<\/mml:mn><mml:mi>\u03b2<\/mml:mi><\/mml:math>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.159<\/mml:mn><\/mml:math>) and TNF-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mrow><mml:mi>\u03b1<\/mml:mi><\/mml:mrow><\/mml:math>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.260<\/mml:mn><\/mml:math>), as compared to IR. The histological score showed no significant differences among the groups. In conclusion, RC-3095 does not demonstrate a protective effect in our LIRI model. Additionally, its use after reperfusion seems to potentiate cell damage, stimulating apoptosis.<\/jats:p>","DOI":"10.1155\/2015\/496378","type":"journal-article","created":{"date-parts":[[2015,3,29]],"date-time":"2015-03-29T17:01:49Z","timestamp":1427648509000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["RC-3095, a Selective Gastrin-Releasing Peptide Receptor Antagonist, Does Not Protect the Lungs in an Experimental Model of Lung Ischemia-Reperfusion Injury"],"prefix":"10.1155","volume":"2015","author":[{"given":"Vera L.","family":"Oliveira-Freitas","sequence":"first","affiliation":[{"name":"Department of Research Group and Post-Graduation, Hospital de Cl\u00ednicas de Porto Alegre, Ramiro Barcelos 2350, Sala 2227, 90.035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Dalla Giacomassa Rocha","family":"Thomaz","sequence":"additional","affiliation":[{"name":"Lung and Airway Laboratory, Federal University of Rio Grande do Sul, Hospital de Cl\u00ednicas de Porto Alegre, Ramiro Barcelos 2350, Sala 21313, 90.035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Elias Lise","family":"Simoneti","sequence":"additional","affiliation":[{"name":"Lung and Airway Laboratory, Federal University of Rio Grande do Sul, Hospital de Cl\u00ednicas de Porto Alegre, Ramiro Barcelos 2350, Sala 21313, 90.035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Malfitano","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Physiology, Universidade Nove de Julho, Rua Vergueiro 235\/249, 3 Subsolo, 01.504-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00e1tia","family":"De Angelis","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Physiology, Universidade Nove de Julho, Rua Vergueiro 235\/249, 3 Subsolo, 01.504-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Maria","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital de Cl\u00ednicas de Porto Alegre, Ramiro Barcelos 2350, 90.035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"Schwartsmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Federal University of Rio Grande do Sul, Hospital de Cl\u00ednicas de Porto Alegre, Rua Ramiro Barcelos 2350, 3\u00b0 Leste, 90.035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano Feij\u00f3","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Hospital de Cl\u00ednicas de Porto Alegre and Hospital da Crian\u00e7a Santo Ant\u00f4nio, Ramiro Barcelos 2.350, 90035-903 Porto 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