{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T22:21:00Z","timestamp":1780611660574,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"CCRUM, Department of AYUSH (Ministry of Health and Family welfare)","award":["3-6\/2009-CCRUM\/EMR"],"award-info":[{"award-number":["3-6\/2009-CCRUM\/EMR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. Hydroalcoholic extract of<jats:italic>Picrorhiza kurroa<\/jats:italic>and its fractions were subjected to<jats:italic>in vitro<\/jats:italic>screening for cytotoxicity; further best active fraction (BAF) obtained was tested against Ehrlich ascites carcinoma (EAC) model in Balb\/c mice after its quality control analysis.<jats:italic>Methods<\/jats:italic>. Cytotoxicities of all the fractions and mother extract of<jats:italic>P. kurroa<\/jats:italic>were determined, using MTT assay on breast cancer (MCF-7, MDA-MB 231) and cervical cancer (HeLa, SiHa) cell lines. Metabolic fingerprinting was developed using HPTLC with quantification of biomarkers (cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin) in BAF. The EAC tumor-bearing mice were used for<jats:italic>in vivo<\/jats:italic>anticancer activity after oral administration (50\u2009mg\u2009Kg<jats:sup>\u22121<\/jats:sup>) for 10 days.<jats:italic>Results<\/jats:italic>. Cytotoxicity assay of mother extract and its fractions over breast cancer and cervix cancer cell lines showed that dichloromethane (DCM) fraction was most cytotoxic (IC<jats:sub>50<\/jats:sub>36.0\u201351.0\u2009<jats:italic>\u00b5<\/jats:italic>g\u2009mL<jats:sup>\u22121<\/jats:sup>at 72\u2009h). Oral administration of DCM fraction showed significant reduction in tumor regression parameters, viable tumor cell count and restoration of hematological parameters may be due to presence of cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin, as compared to the untreated mice of the control group.<jats:italic>Conclusion<\/jats:italic>. The DCM fraction of<jats:italic>P. kurroa<\/jats:italic>displayed potent anticancer activity and can be further explored for the development of a potential candidate for cancer therapy.<\/jats:p>","DOI":"10.1155\/2015\/513875","type":"journal-article","created":{"date-parts":[[2015,10,18]],"date-time":"2015-10-18T17:02:00Z","timestamp":1445187720000},"page":"1-18","source":"Crossref","is-referenced-by-count":33,"title":["HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of<i>Picrorhiza kurroa<\/i>"],"prefix":"10.1155","volume":"2015","author":[{"given":"Md. 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