{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:31:26Z","timestamp":1790847086411,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Wroclaw Research Center EIT+","award":["POIG 01.01.02-02-003\/08-00"],"award-info":[{"award-number":["POIG 01.01.02-02-003\/08-00"]}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["POIG 01.01.02-02-003\/08-00"],"award-info":[{"award-number":["POIG 01.01.02-02-003\/08-00"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Targeting Nampt\/PBEF\/visfatin is considered a promising anticancer strategy, yet little is known about its association with colorectal cancer (CRC). We quantified Nampt\/PBEF\/visfatin expression in bowel and blood (mRNA and protein), referring it to CRC advancement and inflammatory, angiogenic, hypoxia, and proliferation indices. Tumor Nampt\/PBEF\/visfatin upregulation was associated with metastasis, anemia, tumor location,<jats:italic>HIF1<jats:italic>\u03b1<\/jats:italic><\/jats:italic>, and inflammatory and angiogenic indices, of which<jats:italic>HIF1<jats:italic>\u03b1<\/jats:italic><\/jats:italic>,<jats:italic>IL1<jats:italic>\u03b2<\/jats:italic><\/jats:italic>, and anemia explained 70% in Nampt\/PBEF\/visfatin variability. Nampt\/PBEF\/visfatin expression in nontumor tissue, both mRNA and protein, increased in patients with metastatic disease and mild anemia, and, on transcriptional level, correlated with<jats:italic>HIF1<jats:italic>\u03b1<\/jats:italic><\/jats:italic>,<jats:italic>IL1<jats:italic>\u03b2<\/jats:italic><\/jats:italic>,<jats:italic>IL8<\/jats:italic>,<jats:italic>CCL2<\/jats:italic>, and<jats:italic>CCL4<\/jats:italic>expression. Whole blood Nampt\/PBEF\/visfatin tended to be elevated in patients with metastatic cancer or anemia and correlated with inflammatory indices, of which<jats:italic>IL1<jats:italic>\u03b2<\/jats:italic><\/jats:italic>,<jats:italic>IL8<\/jats:italic>, and hematocrit explained 60% of its variability. Circulating visfatin was associated with lymph node metastasis and inflammatory and angiogenic indices.<jats:italic>In vitro<\/jats:italic>experiments on SW620 cells demonstrated Nampt\/PBEF\/visfatin downregulation in response to serum withdrawal but its upregulation in response to serum induction and hypoxia. Stimulation with recombinant visfatin did not provide growth advantage. Summarizing, our results link Nampt\/PBEF\/visfatin with tumor metastatic potential and point at inflammation and hypoxia as key inducers of its upregulation in CRC.<\/jats:p>","DOI":"10.1155\/2015\/523930","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T21:08:03Z","timestamp":1431551283000},"page":"1-12","source":"Crossref","is-referenced-by-count":37,"title":["Nampt\/PBEF\/Visfatin Upregulation in Colorectal Tumors, Mirrored in Normal Tissue and Whole Blood of Colorectal Cancer Patients, Is Associated with Metastasis, Hypoxia, IL1<i>\u03b2<\/i>, and Anemia"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3650-9311","authenticated-orcid":true,"given":"Katarzyna","family":"Neubauer","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Bednarz Misa","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1377-5601","authenticated-orcid":true,"given":"Dorota","family":"Diakowska","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and General Surgery, Wroclaw Medical University, M. Curie-Sklodowskiej 66, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Kapturkiewicz","sequence":"additional","affiliation":[{"name":"First Department of Oncological Surgery, Lower Silesian Oncology Center, Plac Hirszfelda 12, 53-413 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Gamian","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland"},{"name":"Wroclaw Research Center EIT+, Stablowicka 147, 54-066 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Krzystek-Korpacka","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2174\/138161209787185814"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.10.2.12581"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1021\/jm4001049"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2013.85"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(99)01578-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10584"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2010.01518.x"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2013.02.012"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1111\/ajco.12090"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0807581"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1097\/mib.0000000000000067"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.05.033"},{"issue":"7","key":"13","volume":"3","year":"2002","journal-title":"Genome Biology"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.10.001"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2013.11.003"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.ccr-13-0150"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3892\/or.2011.1378"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m112.357301"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069963"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1172\/jci200419930"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m805866200"},{"issue":"7","key":"22","doi-asserted-by":"crossref","first-page":"4685","DOI":"10.4049\/jimmunol.181.7.4685","volume":"181","year":"2008","journal-title":"Journal of Immunology"},{"issue":"3","key":"23","first-page":"399","volume":"19","year":"2010","journal-title":"Advances in Clinical and Experimental Medicine"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.3.1748"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm111"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.12.042"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.06.052"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.07.083"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1007\/s10637-007-9083-2"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00091.2011"},{"issue":"3","key":"31","first-page":"707","volume":"37","year":"2010","journal-title":"International Journal of Oncology"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-012-1476-9"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/523930.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/523930.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/523930.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T06:27:33Z","timestamp":1589524053000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2015\/523930\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":32,"alternative-id":["523930","523930"],"URL":"https:\/\/doi.org\/10.1155\/2015\/523930","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}