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Three <jats:italic>K. pneumoniae<\/jats:italic> clinical isolates were selected for the study with resistance profiles for third\u2010generation cephalosporins, aztreonam, and\/or imipenem and with different resistance genes for extended\u2010spectrum <jats:italic>\u03b2<\/jats:italic>\u2010lactamases (ESBL) or <jats:italic>Klebsiella pneumoniae<\/jats:italic> carbapenemase (KPC). Two <jats:italic>K. pneumoniae<\/jats:italic> isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub\u2010MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The <jats:italic>K. pneumoniae<\/jats:italic> isolates showed different morphological and ultrastructural changes after subjection to <jats:italic>\u03b2<\/jats:italic>\u2010lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that <jats:italic>K. pneumoniae<\/jats:italic> isolates harboring different genes that encode for <jats:italic>\u03b2<\/jats:italic>\u2010lactamases show cell alterations when subjected to different <jats:italic>\u03b2<\/jats:italic>\u2010lactam antibiotics, thus suggesting that they possess residual activity <jats:italic>in vitro<\/jats:italic>, despite the phenotypic resistance presented in the isolates analyzed.<\/jats:p>","DOI":"10.1155\/2015\/572128","type":"journal-article","created":{"date-parts":[[2015,9,28]],"date-time":"2015-09-28T21:02:20Z","timestamp":1443474140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Ultrastructural Changes in Clinical and Microbiota Isolates of <i>Klebsiella pneumoniae<\/i> Carriers of Genes <i>bla<\/i><sub>SHV<\/sub>, <i>bla<\/i><sub>TEM<\/sub>, <i>bla<\/i><sub>CTX-M<\/sub>, or <i>bla<\/i><sub>KPC<\/sub> When Subject to <i>\u03b2<\/i>\u2010Lactam Antibiotics"],"prefix":"10.1155","volume":"2015","author":[{"given":"Dyana Leal","family":"Veras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Catarina","family":"de Souza Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grasielle","family":"Vaz da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Gazzoni","family":"Ara\u00fajo Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina Fernandes","family":"de Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Cristina Gomes","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Am\u00e9lia","family":"Vieira Maciel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Sampaio","family":"Feitosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Carlos","family":"Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Andr\u00e9","family":"Brayner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,28]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.02.012"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.48.7.2400-2408.2004"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0350607"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01940.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/41.4.435"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.6.1033"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.49.11.4443-4447.2005"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg486"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-011-9899-z"},{"key":"e_1_2_7_10_2","unstructured":"JacobyG.andBushK. \u03b2-Lactamase Classification and Amino Acid Sequences for TEM SHV and OXA Extended-Spectrum and Inhibitor Resistant Enzymes 2013 http:\/\/www.lahey.org\/studies."},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1097(02)00484-6"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01686-12"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12056"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2693"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis766"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-009-0126-z"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1590\/s0074-02762010000200009"},{"key":"e_1_2_7_18_2","unstructured":"Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing: fifteenth informational supplement Document 2010 no. 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