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KW-13, a novel cationic<jats:italic>\u03b1<\/jats:italic>-helical antibacterial peptide consisting of 13 amino acid residues, was designed and chemically synthesized. The peptide has a net charge of +6 with a total hydrophobic ratio of 38%. The antibacterial experiments revealed that KW-13 strongly inhibited the growth of human pathogenic bacteria with minimal inhibitory concentrations of 4 and 16\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL for<jats:italic>Staphylococcus epidermidis<\/jats:italic>and<jats:italic>Staphylococcus aureus,<\/jats:italic>respectively, while the hemolytic assay showed that this peptide did not destroy human red blood cells<jats:italic>in vitro<\/jats:italic>. Scanning electron microscopy imaging of<jats:italic>Escherichia coli<\/jats:italic>confirmed that KW-13 can damage the membrane of bacterial cells. Thus, this peptide could be a potential candidate for the treatment of infectious diseases.<\/jats:p>","DOI":"10.1155\/2015\/578764","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T21:02:16Z","timestamp":1448485336000},"page":"1-6","source":"Crossref","is-referenced-by-count":10,"title":["Design and Synthesis of a Novel Cationic Peptide with Potent and Broad-Spectrum Antimicrobial Activity"],"prefix":"10.1155","volume":"2015","author":[{"given":"Wen-Ping","family":"Liu","sequence":"first","affiliation":[{"name":"Children\u2019s Hospital of Zhengzhou, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ming","sequence":"additional","affiliation":[{"name":"Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1056\/nejme020106"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.08.014"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.2174\/1568005024605855"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/s0196-9781(01)00641-6"},{"issue":"13","key":"5","first-page":"3052","volume":"53","year":"1993","journal-title":"Cancer Research"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/292246a0"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1080\/07352689709701952"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/s0041-0101(99)00167-1"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90154-q"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/s0196-9781(01)00498-3"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.09.005"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh025"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn823"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2005.08.016"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.04.003"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1002\/psc.908"},{"issue":"1","key":"17","first-page":"31","volume":"2","year":"2007","journal-title":"Open Life Sciences"},{"issue":"3","key":"18","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1399-3011.1990.tb00939.x","volume":"35","year":"1990","journal-title":"International Journal of Peptide and Protein Research"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1181"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.6.3718"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3011.2001.00802.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg563"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80077-2"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01823-0"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/578764.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/578764.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/578764.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T14:55:00Z","timestamp":1748703300000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/578764\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":25,"alternative-id":["578764","578764"],"URL":"https:\/\/doi.org\/10.1155\/2015\/578764","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}