{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T15:29:37Z","timestamp":1750260577893},"reference-count":42,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Guangdong Medical Research Fund of China","award":["B2012026"],"award-info":[{"award-number":["B2012026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Ophthalmology"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Retinopathy of prematurity (ROP) is a serious disease of preterm neonates and there are limited systematic studies of the molecular mechanisms underlying ROP. Therefore, here we performed global gene expression profiling in human fetal retinal microvascular endothelial cells (RMECs) under hypoxic conditions<jats:italic>in vitro<\/jats:italic>. Aborted fetuses were enrolled and primary RMECs were isolated from eyeballs. Cultivated cells were treated with CoCl<jats:sub>2<\/jats:sub>to induce hypoxia. The dual-color microarray approach was adopted to compare gene expression profiling between treated RMECs and the paired untreated control. The one-class algorithm in significance analysis of microarray (SAM) software was used to screen the differentially expressed genes (DEGs) and quantitative RT-PCR (qRT-PCR) was conducted to validate the results. Gene Ontology was employed for functional enrichment analysis. There were 326 DEGs between the hypoxia-induced group and untreated group. Of these genes, 198 were upregulated in hypoxic RMECs, while the other 128 hits were downregulated. In particular, genes in the iron ion homeostasis pathway were highly enriched under hypoxic conditions. Our study indicates that dysregulation of genes involved in iron homeostasis mediating oxidative damage may be responsible for the mechanisms underlying ROP. The \u201coxygen plus iron\u201d hypothesis may improve our understanding of ROP pathogenesis.<\/jats:p>","DOI":"10.1155\/2015\/584854","type":"journal-article","created":{"date-parts":[[2015,10,18]],"date-time":"2015-10-18T21:03:05Z","timestamp":1445202185000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis"],"prefix":"10.1155","volume":"2015","author":[{"given":"Xian-qiong","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou 511000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-bo","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai-hua","family":"Yin","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Guo","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu-zai","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Deng","sequence":"additional","affiliation":[{"name":"Translational Medicine Center, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Translational Medicine Center, Guangdong Women and Children\u2019s Hospital, Guangzhou 511400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2004-1309"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2012.12.038"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2012.195"},{"issue":"8","key":"4","first-page":"3169","volume":"35","year":"1994","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m300463200"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1159\/000315364"},{"key":"7","first-page":"171","volume":"9","year":"2003","journal-title":"Molecular Vision"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400461200"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5510"},{"issue":"8","key":"10","first-page":"2343","volume":"41","year":"2000","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2007.07.004"},{"issue":"2","key":"12","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1542\/peds.72.2.159","volume":"72","year":"1983","journal-title":"Pediatrics"},{"issue":"10","key":"13","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/BF02073381","volume":"152","year":"1993","journal-title":"European Journal of Pediatrics"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-3782(01)00115-3"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/aos.12229"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s004310050641"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1136\/fn.84.3.F188"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1172\/jci448"},{"issue":"13","key":"19","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1096\/fasebj.13.13.1800","volume":"13","year":"1999","journal-title":"The FASEB Journal"},{"issue":"4","key":"20","first-page":"L672","volume":"271","year":"1996","journal-title":"American Journal of Physiology: Lung Cellular and Molecular Physiology"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700691"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1159\/000356649"},{"issue":"3","key":"23","first-page":"744","volume":"40","year":"1999","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2722"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2306"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(96)00483-2"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(92)90091-t"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.20914"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio807"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805361105"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.02.007"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(04)00343-5"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(01)00518-9"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.1.5145"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.24147"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.24142"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.1998.2112"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1172\/jci67225"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.062018"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0026"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1933581100"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2013.867484"}],"container-title":["Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2015\/584854.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2015\/584854.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2015\/584854.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T13:55:40Z","timestamp":1653314140000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/joph\/2015\/584854\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":42,"alternative-id":["584854","584854"],"URL":"https:\/\/doi.org\/10.1155\/2015\/584854","relation":{},"ISSN":["2090-004X","2090-0058"],"issn-type":[{"value":"2090-004X","type":"print"},{"value":"2090-0058","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}