{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T01:15:42Z","timestamp":1783559742273,"version":"3.55.0"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Tuberculosis (TB) remains a major global health problem and is the second biggest cause of death by infectious disease worldwide. Here, we investigate in vitro the Th1, Th2, Th17, and Treg cytokines and transcriptional factors produced after<jats:italic>Mycobacterium<\/jats:italic>-specific antigen stimulation in patients with active pulmonary tuberculosis, clinically cured pulmonary tuberculosis, and healthy donors with a positive tuberculin skin test (TST+). Together, our data indicate that clinical cure after treatment increases the percentages of<jats:italic>Mycobacterium<\/jats:italic>-specific Th1, Th2, and Th17 cells compared with those found in active-TB and TST+ healthy donors. These results show that the host-parasite equilibrium in latent TB breaks in favor of the microorganism and that the subsequent clinical recovery posttreatment does not return the percentage levels of such cells to those observed in latent tuberculosis. Additionally, our results indicate that rather than showing an increase in the percentage of<jats:italic>Mycobacterium<\/jats:italic>-specific Tregs, active-TB patients display lower Th1\u2009:\u2009Treg and Th17\u2009:\u2009Treg ratios. These data, together with lower Th1\u2009:\u2009Th2 and Th17\u2009:\u2009Th2 ratios, may indicate a mechanism by which the breakdown of the host-parasite equilibrium leads to active-TB and changes in the repertoire of<jats:italic>Mycobacterium<\/jats:italic>-specific Th cells that are associated with clinical cure after treatment of pulmonary tuberculosis.<\/jats:p>","DOI":"10.1155\/2015\/591237","type":"journal-article","created":{"date-parts":[[2015,4,27]],"date-time":"2015-04-27T21:04:00Z","timestamp":1430168640000},"page":"1-14","source":"Crossref","is-referenced-by-count":13,"title":["Expression Pattern of Transcription Factors and Intracellular Cytokines Reveals That Clinically Cured Tuberculosis Is Accompanied by an Increase in<i>Mycobacterium<\/i>-Specific Th1, Th2, and Th17 Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"Marcos V.","family":"da Silva","sequence":"first","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir J.","family":"Massaro Junior","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana R.","family":"Machado","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"},{"name":"Department of Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s, 74605-050 Goiania, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djalma A. A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00facio R.","family":"Castellano","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"},{"name":"Human Immunology Research and Education Group (GEPIH), Technical School of Health, Federal University of Para\u00edba, 58051-900 Jo\u00e3o Pessoa, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia B. D.","family":"Alexandre","sequence":"additional","affiliation":[{"name":"Municipal Secretary Office of Health, 38065-160 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise B. R.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"},{"name":"Laboratory of Biopathology and Molecular Biology, University of Uberaba, 38055-500 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virmondes","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Institute of Biological Sciences, Tri\u00e2ngulo Mineiro Federal University, 38025-180 Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","year":"2013"},{"issue":"9947","key":"2","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/S0140-6736(14)60844-8","volume":"384","year":"2014","journal-title":"The Lancet"},{"issue":"11","key":"3","first-page":"889","volume":"89","year":"2014","journal-title":"The American Family Physician"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.93"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065492"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2243"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxr029"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535496"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.07.022"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.00791.x"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1086\/315325"},{"issue":"4","key":"12","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1128\/IAI.64.4.1351-1356.1996","volume":"64","year":"1996","journal-title":"Infection and Immunity"},{"issue":"11","key":"13","doi-asserted-by":"crossref","first-page":"5597","DOI":"10.1128\/IAI.67.11.5597-5603.1999","volume":"67","year":"1999","journal-title":"Infection and Immunity"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.15.2.294-309.2002"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903332"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1995"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100265"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141623"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.01.016"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2006001100002"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03027.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200508-1294oc"},{"key":"23","year":"2013"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4067\/s0034-98872012000800008"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1002\/stem.5530120502"},{"issue":"4","key":"26","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.4049\/jimmunol.143.4.1123","volume":"143","year":"1989","journal-title":"Journal of Immunology"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.6.1988"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990200205"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2002.01858.x"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/s0928-8244(03)00166-4"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.03.020"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1086\/314852"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1086\/515336"},{"issue":"4","key":"34","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.4049\/jimmunol.142.4.1114","volume":"142","year":"1989","journal-title":"The Journal of Immunology"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2005.08.007"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095939"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01321-8"},{"issue":"8","key":"38","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1128\/IAI.63.8.3231-3234.1995","volume":"63","year":"1995","journal-title":"Infection and Immunity"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1086\/344903"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2003.10.007"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2007.03.004"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00602-06"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1128\/iai.69.5.3497-3501.2001"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2012.12.002"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4593"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.03.016"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1086\/605532"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.081501.125851"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-280586"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/285094"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202870109"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101058"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201142240"},{"issue":"2","key":"54","first-page":"171","volume":"81","year":"1994","journal-title":"Immunology"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.11.038"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.10.293"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.06.005"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.788"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1128\/iai.01392-09"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00550-12"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.3.1962"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200909-1463oc"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.3.1237"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1993"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.005"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1038\/ni1467"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1038\/ni1449"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110919"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01018.x"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.009"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172702"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2426"},{"key":"73","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1731"},{"key":"74","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2425"},{"key":"75","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.03.002"},{"key":"76","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.05.003"},{"key":"77","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0908-0"},{"key":"78","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1711.2006.01460.x"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/591237.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/591237.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/591237.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T20:59:01Z","timestamp":1691614741000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/591237\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":78,"alternative-id":["591237","591237"],"URL":"https:\/\/doi.org\/10.1155\/2015\/591237","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}