{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:20:07Z","timestamp":1761060007674},"reference-count":46,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Research Foundation of E-DA Hospital","award":["EDAHP-103050","EDAHP-103035","NCKUEDA- 1030","NCKUEDA-10402"],"award-info":[{"award-number":["EDAHP-103050","EDAHP-103035","NCKUEDA- 1030","NCKUEDA-10402"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Peritoneal dialysis (PD) can induce fibrosis and functional alterations in PD patients\u2019 peritoneal membranes, due to long-term unphysiological dialysate exposure, partially occurring via triggering of epithelial-to-mesenchymal transition (EMT) in peritoneal mesothelial cells (MCs). Vitamin D can ameliorate these negative effects; however, the mechanism remains unexplored. Therefore, we investigated its possible links to MCs EMT inhibition.<jats:italic>Methods<\/jats:italic>. Peritoneal fibrosis was established in Sprague-Dawley rats by chlorhexidine gluconate (CG) intraperitoneal injection for 21 days, with and without 1<jats:italic>\u03b1<\/jats:italic>,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>treatment. Morphological and functional evaluation and western blot analysis of EMT marker were performed upon peritoneum tissue.<jats:italic>In vitro<\/jats:italic>study was also performed in a primary human peritoneal MC culture system; MCs were incubated with transforming growth factor-<jats:italic>\u03b2<\/jats:italic>1 (TGF-<jats:italic>\u03b2<\/jats:italic>1) in the absence or presence of 1<jats:italic>\u03b1<\/jats:italic>,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>. EMT marker expression, migration activities, and cytoskeleton redistribution of MCs were determined.<jats:italic>Results<\/jats:italic>. 1<jats:italic>\u03b1<\/jats:italic>,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>ameliorated CG-induced morphological and functional deterioration in animal model, along with CG-induced upregulation of<jats:italic>\u03b1<\/jats:italic>-SMA and downregulation of E-cadherin expression. Meanwhile, 1<jats:italic>\u03b1<\/jats:italic>,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>also ameliorated TGF-<jats:italic>\u03b2<\/jats:italic>1-induced decrease in E-cadherin expression, increase in Snai1 and<jats:italic>\u03b1<\/jats:italic>-SMA expression, intracellular F-actin redistribution, and migration activity<jats:italic>in vitro<\/jats:italic>.<jats:italic>Conclusion<\/jats:italic>. 1<jats:italic>\u03b1<\/jats:italic>,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>can ameliorate CG-induced peritoneal fibrosis and attenuate functional deterioration through inhibiting MC EMT.<\/jats:p>","DOI":"10.1155\/2015\/595030","type":"journal-article","created":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T21:17:51Z","timestamp":1443734271000},"page":"1-12","source":"Crossref","is-referenced-by-count":6,"title":["Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"Yi-Che","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, E-DA Hospital, I-Shou University, Kaohsiung, Taiwan"},{"name":"Institute of Clinical Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Yuan","family":"Hung","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, E-DA Hospital, I-Shou University, Kaohsiung, Taiwan"},{"name":"School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung-Hsiang","family":"Liou","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Hsin-Jen Hospital, New Taipei City, 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