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These diseases include viral hepatitis, liver fibrosis, cardiac arrhythmia, skin diseases, and tumors. However, matrine is also the main toxic compound of this herb, and the available biomarkers are not reliable in detecting or quantifying matrine risk. Metabolomics is a powerful tool used to identify early toxicity biomarkers that are specific indicators of damage to biosystems. This study aimed to find the potential biomarkers of the matrine-induced toxic effects in rats and HepG2 cells. The toxicological effects of rats induced by matrine could be derived from the elevated taurine and trimethylamine N-oxide levels and the depletion in hippurate and tricarboxylic acid cycle intermediates, such as 2-oxoglutarate, citrate, and succinate in the urine. Cell metabolomics revealed that the levels of alanine, choline, glutathione, lactate, phosphocholine, and cholesterol showed dose-dependent decreases, whereas the levels of taurine, fatty acid, and unsaturated fatty acid showed dose-dependent increases. Overall, a significant perturbation of metabolites in response to high dose of matrine was observed both<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>, and the selected metabolites particularly represent an attractive marker for matrine-induced toxicity.<\/jats:p>","DOI":"10.1155\/2015\/598412","type":"journal-article","created":{"date-parts":[[2015,8,30]],"date-time":"2015-08-30T21:29:53Z","timestamp":1440970193000},"page":"1-11","source":"Crossref","is-referenced-by-count":23,"title":["Toxic Markers of Matrine Determined Using<sup><b>1<\/b><\/sup>H-NMR-Based Metabolomics in Cultured Cells<i>In Vitro<\/i>and Rats<i>In Vivo<\/i>"],"prefix":"10.1155","volume":"2015","author":[{"given":"Zhonghuang","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, China"},{"name":"International Institute for Translational Chinese Medicine, Guangzhou University of Chinese 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