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The role of many identified miRNAs is not yet resolved. Recent evidence suggests that miRNA mutations and\/or misexpression may contribute to genetic disorders. Point mutations in the seed region of<jats:italic>MIR184<\/jats:italic>have been recently identified in Keratoconus (KC) patients with or without other corneal and lens abnormalities. We investigated mutations within<jats:italic>MIR184<\/jats:italic>in KC patients from Saudi Arabia and examined the relative expression of miR-184 and miR-205 in human cornea. Ethnically matched KC cases (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>134<\/mml:mn><\/mml:math>) were recruited and sequencing was performed using PCR-based Sanger sequencing and analyzed using the Sequencher 5.2 software. Expression of miR-184 and miR-205 was profiled in postmortem unaffected ocular tissues obtained from donors with no history of ocular diseases. miR-184 expression was 15-fold higher than that of miR-205 in cornea samples. No mutation(s) within the screened genomic region of<jats:italic>MIR184<\/jats:italic>in KC cases was detected. This suggests that mutation in<jats:italic>MIR184<\/jats:italic>is a rare cause of KC alone and may be more relevant to cases of KC associated with other ocular abnormalities. The increased expression of miR-184 versus miR-205 in normal cornea samples implies a possible role of miR184 in cornea development and\/or corneal diseases.<\/jats:p>","DOI":"10.1155\/2015\/604508","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T17:26:30Z","timestamp":1440437190000},"page":"1-7","source":"Crossref","is-referenced-by-count":27,"title":["Screening of the Seed Region of<i>MIR184<\/i>in Keratoconus Patients from Saudi Arabia"],"prefix":"10.1155","volume":"2015","author":[{"given":"Khaled K.","family":"Abu-Amero","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inas","family":"Helwa","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, the Medical College of Georgia, Georgia Regents University, Augusta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman","family":"Al-Muammar","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelby","family":"Strickland","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Hauser","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, NC, USA"},{"name":"Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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