{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:44:11Z","timestamp":1792183451713,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T00:00:00Z","timestamp":1450742400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Medicine and Pharmacy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Bacteriology"],"published-print":{"date-parts":[[2015,12,22]]},"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic>is the causative agent of melioidosis. The complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. In various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as RpoS and RpoN to modulate genes expression for their adaptation and survival. In this study, we demonstrate that mutagenesis of<jats:italic>rpoN2<\/jats:italic>, which lies on chromosome 2 of<jats:italic>B. pseudomallei<\/jats:italic>and encodes a homologue of the sigma factor RpoN, did not alter nitrogen and amino acid utilization of the bacterium. However, introduction of<jats:italic>B. pseudomallei rpoN2<\/jats:italic>into<jats:italic>E. coli<\/jats:italic>strain deficient for<jats:italic>rpoN<\/jats:italic>restored the ability to utilize amino acids. Moreover, comparative partial proteomic analysis of the<jats:italic>B. pseudomallei<\/jats:italic>wild type and its<jats:italic>rpoN2<\/jats:italic>isogenic mutant was performed to elucidate its amino acids utilization property which was comparable to its function found in the complementation assay. By contrast, the<jats:italic>rpoN2<\/jats:italic>mutant exhibited decreased<jats:italic>katE<\/jats:italic>expression at the transcriptional and translational levels. Our finding indicates that<jats:italic>B. pseudomallei<\/jats:italic>RpoN2 is involved in a specific function in the regulation of catalase E expression.<\/jats:p>","DOI":"10.1155\/2015\/623967","type":"journal-article","created":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T21:02:35Z","timestamp":1450818155000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Role of<i>Burkholderia pseudomallei<\/i>Sigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level"],"prefix":"10.1155","volume":"2015","author":[{"given":"Duong Thi Hong","family":"Diep","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Thanh","family":"Phuong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mya Myintzu","family":"Hlaing","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Salaya 73170, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Potjanee","family":"Srimanote","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Salaya 73170, Thailand"},{"name":"Graduate Programme in Biomedical Sciences, Faculty of Allied Health Science, Thammasat University, Rungsit 12121, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumalee","family":"Tungpradabkul","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, 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