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This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or filter-sterilized PD fluids. Data from selected heat-shock proteins were validated by 2D western-blot analysis. Comparison of proteomics and transcriptomics data discriminated differentially regulated protein abundance into groups depending on correlating or noncorrelating transcripts. Inadequate abundance of several heat-shock proteins following exposure to heat-sterilized PD fluids is not reflected on the mRNA level indicating interference beyond transcriptional regulation. For the first time, this study describes evidence for posttranscriptional inadequacy of heat-shock protein expression by heat-sterilized PD fluids as a novel cytotoxic property. Cross-omics technologies introduce a novel way of understanding PDF bioincompatibility and searching for new interventions to reestablish adequate cytoprotective responses.<\/jats:p>","DOI":"10.1155\/2015\/628158","type":"journal-article","created":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T21:15:59Z","timestamp":1443734159000},"page":"1-12","source":"Crossref","is-referenced-by-count":4,"title":["Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids"],"prefix":"10.1155","volume":"2015","author":[{"given":"Klaus","family":"Kratochwill","sequence":"first","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria"},{"name":"Zytoprotec GmbH, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten O.","family":"Bender","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and 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