{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:34:45Z","timestamp":1771457685766,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Serum desmoglein enzyme-linked immunosorbent assay (ELISA) is used for the diagnosis and monitoring of pemphigus diseases.<jats:italic>Objectives<\/jats:italic>. To compare the diagnostic accuracy of salivary antidesmoglein (Dsg) 1 and 3 ELISA in the diagnosis of pemphigus vulgaris (PV) patients with that of serum desmogleins ELISA.<jats:italic>Methods<\/jats:italic>. Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study. PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings. After processing, serum and salivary anti-Dsg 1 and 3 were measured by the ELISA method using Euroimmun kit (L\u00fcbeck, Germany).<jats:italic>Results<\/jats:italic>. Using the cut-off point of 20 relative units (RU)\/mL, the serum anti-Dsg 1 and 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) patients, respectively, and the salivary anti-Dsg 1 and 3 ELISA were positive in 31 (36.1%) and 63 (73.3%) patients, respectively. The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%. Optimal cut-off values of 7.7 and 13.4\u2009RU\/mL were determined for the salivary anti-Dsg 1 and anti-Dsg 3 ELISA, respectively.<jats:italic>Conclusion<\/jats:italic>. Salivary anti-Dsg 1 and 3 ELISA with high specificities (98.9%) could be suggested as safe and noninvasive methods for the diagnosis of PV when obtaining a blood sample is difficult.<\/jats:p>","DOI":"10.1155\/2015\/698310","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T16:01:22Z","timestamp":1421942482000},"page":"1-7","source":"Crossref","is-referenced-by-count":12,"title":["Salivary Desmoglein Enzyme-Linked Immunosorbent Assay for Diagnosis of Pemphigus Vulgaris: A Noninvasive Alternative Test to Serum Assessment"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3530-3541","authenticated-orcid":true,"given":"Hossein","family":"Mortazavi","sequence":"first","affiliation":[{"name":"Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran 1199663911, Iran"},{"name":"Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Vahdat Islamic Square, Tehran 1199663911, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Khatami","sequence":"additional","affiliation":[{"name":"Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran 1416613675, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Seyedin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Vahdat Islamic Square, Tehran 1199663911, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2273-303X","authenticated-orcid":true,"given":"Iman","family":"Vasheghani Farahani","sequence":"additional","affiliation":[{"name":"Department of Industrial and Systems Engineering, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Daneshpazhooh","sequence":"additional","affiliation":[{"name":"Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran 1199663911, Iran"},{"name":"Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Vahdat Islamic Square, Tehran 1199663911, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.02752.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2011.07.004"},{"issue":"1","key":"3","first-page":"53","volume":"8","year":"2009","journal-title":"Iranian Journal of Allergy, Asthma and Immunology"},{"issue":"4","key":"4","first-page":"241","volume":"22","year":"2009","journal-title":"American Journal of Dentistry"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03408.x"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.80406"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2003.438"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70183-0"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.07.006"},{"issue":"2","key":"10","first-page":"124","volume":"2","year":"2009","journal-title":"Journal of Medicine and Life"},{"issue":"3","key":"11","first-page":"287","volume":"10","year":"2013","journal-title":"Dental Research Journal"},{"issue":"7","key":"12","first-page":"651","volume":"127","year":"2010","journal-title":"Texas Dental Journal"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1384.012"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2008.02715.x"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00146-9"},{"key":"16","first-page":"3","volume-title":"Studies and systemic reviews on diagnostic test accuracy","year":"2011"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2000.00624.x"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2007.02254.x"},{"key":"19","first-page":"31","volume-title":"Diagnosis and screening","year":"2002"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-4356(03)00177-X"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04838.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2010.01069.x"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2011.04294.x"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2012.1901"},{"issue":"2","key":"25","first-page":"143","volume":"135","year":"1999","journal-title":"Archives of Dermatology"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/698310.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/698310.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/698310.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T19:03:17Z","timestamp":1469646197000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/698310\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":26,"alternative-id":["698310","698310"],"URL":"https:\/\/doi.org\/10.1155\/2015\/698310","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}