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We investigated the effect of branched polyethylenimine-superparamagnetic iron oxide nanoparticles (bPEI-SPIONs) on tumor cells loaded onto DCs. The tumor antigens were prepared as follows: (1) apoptotic U266 cells with ultraviolet B (UVB) irradiation followed by a 2\u2009h incubation in the absence (2\u2009h postirradiated cells) or (2) presence of bPEI-SPIONs (bPEI-SPION 2\u2009h postirradiated cells) and (3) apoptotic U266 cells with UVB irradiation followed by an overnight 16\u2009h incubation (16\u2009h postirradiated cells). bPEI-SPIONs render U266 cells sensitive to UVB irradiation through reactive oxygen species production to accelerate apoptotic death. The 2\u2009h postirradiated cells and bPEI-SPION 2\u2009h postirradiated cells released immunogenic proteins, including Hsp70, Hsp90, and HMGB1. The DCs loaded with bPEI-SPION 2\u2009h postirradiated cells showed the highest IL-12p70 production and Th1 polarization compared with other DCs. These results suggest that bPEI-SPIONs are a promising method of enhancing the immunogenicity of tumor cells and promoting Th1 polarization of DCs loaded with these tumor cells.<\/jats:p>","DOI":"10.1155\/2015\/706379","type":"journal-article","created":{"date-parts":[[2015,6,28]],"date-time":"2015-06-28T17:02:50Z","timestamp":1435510970000},"page":"1-9","source":"Crossref","is-referenced-by-count":28,"title":["Branched Polyethylenimine-Superparamagnetic Iron Oxide Nanoparticles (bPEI-SPIONs) Improve the Immunogenicity of Tumor Antigens and Enhance Th1 Polarization of Dendritic Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"My-Dung","family":"Hoang","sequence":"first","affiliation":[{"name":"Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 519-763, Republic of Korea"},{"name":"Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 519-763, Republic of 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