{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:48:38Z","timestamp":1792799318375,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of State for Scientific Research","award":["5575"],"award-info":[{"award-number":["5575"]}],"id":[{"id":"https:\/\/ror.org\/01r2j1v82","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    . Developing a validated HPLC-DAD method for simultaneous determination of posaconazole (PSZ) and vincristine (VCR) in rat plasma.\n                    <jats:italic>Methods<\/jats:italic>\n                    . PSZ, VCR, and itraconazole (ITZ) were extracted from 200\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    L plasma using diethyl ether in the presence of 0.1\u2009M sodium hydroxide solution. The organic layer was evaporated in vacuo and dried residue was reconstituted and injected through HC-C18 (4.6 \u00d7 250\u2009mm, 5\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    m) column. In the mobile phase, acetonitrile and 0.015\u2009M potassium dihydrogen orthophosphate (30\u2009:\u200970 to 80\u2009:\u200920, linear gradient over 7 minutes) pumped at 1.5\u2009mL\/min. VCR and PSZ were measured at 220 and 262\u2009nm, respectively. Two Sprague Dawley rats were orally dosed PSZ followed by iv dosing of VCR and serial blood sampling was performed.\n                    <jats:italic>Results<\/jats:italic>\n                    . VCR, PSZ, and ITZ were successfully separated within 11\u2009min. Calibration curves were linear over the range of 50\u20135000\u2009ng\/mL for both drugs. The CV% and % error of the mean were \u226418% and limit of quantitation was 50\u2009ng\/mL for both drugs. Rat plasma concentrations of PSZ and VCR were simultaneously measured up to 72\u2009h and their calculated pharmacokinetics parameters were comparable to the literature.\n                    <jats:italic>Conclusion.<\/jats:italic>\n                    The assay was validated as per ICH guidelines and is appropriate for pharmacokinetics drug-drug interaction studies.\n                  <\/jats:p>","DOI":"10.1155\/2015\/743915","type":"journal-article","created":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T16:03:12Z","timestamp":1450800192000},"page":"1-6","source":"Crossref","is-referenced-by-count":11,"title":["High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma"],"prefix":"10.1155","volume":"2015","author":[{"given":"Hadeel A.","family":"Khalil","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Alexandria University, Alexandria 21521, 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