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In this study, we determined the ability of AcNPV-LC3 to protect against ALA by the intramuscular route as well as the liver immune response associated with protection. Results showed that 55% of hamsters IM immunized with AcNPV-LC3 showed sterile protection against ALA, whereas other 20% showed reduction in the size and extent of abscesses, resulting in some protection in 75% of animals compared to the sham control group. Levels of protection showed a linear correlation with the development and intensity of specific antiamoeba cellular and humoral responses, evaluated in serum and spleen of hamsters, respectively. Evaluation of the Th1\/Th2 cytokine patterns expressed in the liver of hamsters showed that sterile protection was associated with the production of high levels of IFN<jats:italic>\u03b3<\/jats:italic>and IL-4. These results suggest that the baculovirus system is equally efficient by the intramuscular as well as the oral routes for ALA protection and that the Gal-lectin LC3 fragment is a highly protective antigen against hepatic amoebiasis through the local induction of IFN<jats:italic>\u03b3<\/jats:italic>and IL-4.<\/jats:p>","DOI":"10.1155\/2015\/760598","type":"journal-article","created":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T21:04:50Z","timestamp":1432069490000},"page":"1-10","source":"Crossref","is-referenced-by-count":7,"title":["Protection against Amoebic Liver Abscess in Hamster by Intramuscular Immunization with an<i>Autographa californica<\/i>Baculovirus Driving the Expression of the Gal-Lectin LC3 Fragment"],"prefix":"10.1155","volume":"2015","author":[{"given":"Dulce Mar\u00eda","family":"Meneses-Ruiz","sequence":"first","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de 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