{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:23:12Z","timestamp":1790378592414,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100006054","name":"Universidad de Guanajuato","doi-asserted-by":"publisher","award":["UGTO-id202\/2013"],"award-info":[{"award-number":["UGTO-id202\/2013"]}],"id":[{"id":"10.13039\/501100006054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>\n                    The aims of the study were to evaluate the influence of culture media on biofilm formation by\n                    <jats:italic>C. albicans, C. glabrata, C. krusei,<\/jats:italic>\n                    and\n                    <jats:italic>C. parapsilosis<\/jats:italic>\n                    and to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, the\n                    <jats:italic>Candida<\/jats:italic>\n                    species were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay.\n                    <jats:italic>C. albicans<\/jats:italic>\n                    formed biofilms preferentially in YPD containing 2% glucose (YPD\/2%),\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    in glucose-free YNB or supplemented with 0.2% glucose (YNB\/0.2%), while\n                    <jats:italic>C. krusei<\/jats:italic>\n                    and\n                    <jats:italic>C. parapsilosis<\/jats:italic>\n                    preferred YP, YPD\/0.2%, and YPD\/2%. Interestingly, only\n                    <jats:italic>C. albicans<\/jats:italic>\n                    produced an exopolymeric matrix. This is the first report dealing with the\n                    <jats:italic>in vitro<\/jats:italic>\n                    effect of the culture medium and glucose on the formation of biofilms in four\n                    <jats:italic>Candida<\/jats:italic>\n                    species as well as the resistance of sessile cells to antifungals, AgNPs, and ROS. Our results suggest that candidiasis\n                    <jats:italic>in vivo<\/jats:italic>\n                    is a multifactorial and complex process where the nutritional conditions, the human immune system, and the adaptability of the pathogen should be considered altogether to provide an effective treatment of the patient.\n                  <\/jats:p>","DOI":"10.1155\/2015\/783639","type":"journal-article","created":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T16:04:06Z","timestamp":1422806646000},"page":"1-15","source":"Crossref","is-referenced-by-count":51,"title":["Influence of Culture Media on Biofilm Formation by\n                    <i>Candida<\/i>\n                    Species and Response of Sessile Cells to Antifungals and Oxidative Stress"],"prefix":"10.1155","volume":"2015","author":[{"given":"Isela","family":"Serrano-Fujarte","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Campus Guanajuato, Noria Alta S\/N, 36050 Guanajuato, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2016-7399","authenticated-orcid":true,"given":"Everardo","family":"L\u00f3pez-Romero","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Campus Guanajuato, Noria Alta S\/N, 36050 Guanajuato, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina Elena","family":"Reyna-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Campus Guanajuato, Noria Alta S\/N, 36050 Guanajuato, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma. 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