{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:08:38Z","timestamp":1762506518411,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004515","name":"Universiti Kebangsaan Malaysia","doi-asserted-by":"publisher","award":["DPP-2014-060 and NND\/ND\/2\/TD11-009"],"award-info":[{"award-number":["DPP-2014-060 and NND\/ND\/2\/TD11-009"]}],"id":[{"id":"10.13039\/501100004515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Sensors"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Green fluorescent protein (GFP) is suitable as a toxicity sensor due to its ability to work alone without cofactors or substrates. Its reaction with toxicants can be determined with fluorometric approaches. GFP mutant gene (C48S\/S147C\/Q204C\/S65T\/Q80R) is used because it has higher sensitivity compared to others GFP variants. A novel sodium dodecyl sulfate (SDS) toxicity detection biosensor was built by immobilizing GFP expressing<jats:italic>Escherichia coli<\/jats:italic>in<jats:italic>k<\/jats:italic>-Carrageenan matrix. Cytotoxicity effect took place in the toxicity biosensor which leads to the decrease in the fluorescence intensity. The fabricated<jats:italic>E. coli<\/jats:italic>GFP toxicity biosensor has a wide dynamic range of 4\u2013100\u2009ppm, with LOD of 1.7\u2009ppm. Besides, it possesses short response time (&lt;1\u2009min), high reproducibility (0.76% RSD) and repeatability (0.72% RSD,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:msup><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn>2<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mo>&gt;<\/mml:mo><mml:mn>0.98<\/mml:mn><\/mml:math>), and long-term stability (46 days).<jats:italic>E. coli<\/jats:italic>GFP toxicity biosensor has been applied to detect toxicity induced by SDS in tap water, river water, and drinking water. High recovery levels of SDS indicated the applicability of<jats:italic>E. coli<\/jats:italic>GFP toxicity biosensor in real water samples toxicity evaluation.<\/jats:p>","DOI":"10.1155\/2015\/809065","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T22:39:17Z","timestamp":1426631957000},"page":"1-9","source":"Crossref","is-referenced-by-count":12,"title":["Toxicity Biosensor for Sodium Dodecyl Sulfate Using Immobilized Green Fluorescent Protein Expressing<i>Escherichia coli<\/i>"],"prefix":"10.1155","volume":"2015","author":[{"given":"Lia","family":"Ooi","sequence":"first","affiliation":[{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), LESTARI, National University of Malaysia, 43600 Bangi, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee Yook","family":"Heng","sequence":"additional","affiliation":[{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), 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