{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:02:39Z","timestamp":1784120559244,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Polish Ministry of Science and Higher Education","award":["N N402 591240"],"award-info":[{"award-number":["N N402 591240"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["N N402 375838"],"award-info":[{"award-number":["N N402 375838"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["2012\/05\/B\/NZ7\/02502"],"award-info":[{"award-number":["2012\/05\/B\/NZ7\/02502"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["502-54-127"],"award-info":[{"award-number":["502-54-127"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["N N402 591240"],"award-info":[{"award-number":["N N402 591240"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["N N402 375838"],"award-info":[{"award-number":["N N402 375838"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["2012\/05\/B\/NZ7\/02502"],"award-info":[{"award-number":["2012\/05\/B\/NZ7\/02502"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["502-54-127"],"award-info":[{"award-number":["502-54-127"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["N N402 591240"],"award-info":[{"award-number":["N N402 591240"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["N N402 375838"],"award-info":[{"award-number":["N N402 375838"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["2012\/05\/B\/NZ7\/02502"],"award-info":[{"award-number":["2012\/05\/B\/NZ7\/02502"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["502-54-127"],"award-info":[{"award-number":["502-54-127"]}]},{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["N N402 591240"],"award-info":[{"award-number":["N N402 591240"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["N N402 375838"],"award-info":[{"award-number":["N N402 375838"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["2012\/05\/B\/NZ7\/02502"],"award-info":[{"award-number":["2012\/05\/B\/NZ7\/02502"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["502-54-127"],"award-info":[{"award-number":["502-54-127"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>The aim of presented work was to analyze the impact of particular polymorphic changes in the promoter regions of the -1607 1G\/2G<jats:italic>MMP1<\/jats:italic>, -1562 C\/T<jats:italic>MMP9<\/jats:italic>, -82 A\/G<jats:italic>MMP12<\/jats:italic>, -511 C\/T<jats:italic>IL-1<jats:italic>\u03b2<\/jats:italic><\/jats:italic>, and 372 T\/C<jats:italic>TIMP1<\/jats:italic>genes on their expression level in POAG patients. Blood and aqueous humor samples acquired from 50 patients with POAG and 50 control subjects were used for QPCR and protein levels analysis by ELISA.<jats:italic>In vivo<\/jats:italic>promoter activity assays were carried on HTM cells using dual luciferase assay. All studied subjects underwent ophthalmic examination, including BCVA, intraocular pressure, slit-lamp examination, gonioscopy, HRT, and OCT scans. Patients with POAG are characterized by an increased mRNA expression of<jats:italic>MMP1<\/jats:italic>,<jats:italic>MMP9<\/jats:italic>,<jats:italic>MMP12<\/jats:italic>, and<jats:italic>IL-1<jats:italic>\u03b2<\/jats:italic><\/jats:italic>genes as compared to the control group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>). Aqueous humor acquired from patients with POAG displayed increased protein expression of MMP1, MMP9, MMP12, and IL-1<jats:italic><jats:italic>\u03b2<\/jats:italic><\/jats:italic>compared to the control group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>). Allele -1607 1G of<jats:italic>MMP1<\/jats:italic>gene possesses only 42,91% of the -1607 2G allele transcriptional activity and allele -1562 C of<jats:italic>MMP9<\/jats:italic>gene possesses only 21,86% of the -1562 T allele. Increased expression levels of metalloproteinases can be considered as a risk factor for the development of POAG.<\/jats:p>","DOI":"10.1155\/2015\/812503","type":"journal-article","created":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T17:07:30Z","timestamp":1431364050000},"page":"1-8","source":"Crossref","is-referenced-by-count":50,"title":["Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mo mathvariant=\"bold\">\u03b2<\/mml:mo><\/mml:mrow><\/mml:math>as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients"],"prefix":"10.1155","volume":"2015","author":[{"given":"Lukasz","family":"Markiewicz","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-647 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dariusz","family":"Pytel","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, AFCRI, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA"},{"name":"Department of Biochemistry and Molecular Biology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Mucha","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-647 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Szymanek","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Medical University of Warsaw, 03-709 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy","family":"Szaflik","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Medical University of Warsaw, 03-709 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek P.","family":"Szaflik","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Medical University of Warsaw, 03-709 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ireneusz","family":"Majsterek","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-647 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.86.2.238"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081224"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2010.01.018"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.6.701"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.01.013"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2010.02455.x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2012.09.001"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2012.10.009"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1097\/01.ijg.0000212229.57922.72"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1097\/01.ijg.0000145812.39224.0a"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0365"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/aos.12149"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057955"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/s1350-9462(99)00017-8"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(200011)32:2lt;122::aid-glia20>3.0.co;2-j"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.118.5.666"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-006-0440-1"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(200103)33:3lt;205::aid-glia1019>3.0.co;2-d"},{"key":"19","first-page":"1764","volume":"16","year":"2010","journal-title":"Molecular Vision"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m204824200"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0832"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0198oc"},{"key":"23","first-page":"431","volume":"18","year":"2012","journal-title":"Molecular Vision"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-11-99"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m107865200"},{"issue":"23","key":"26","first-page":"5321","volume":"58","year":"1998","journal-title":"Cancer Research"},{"issue":"7","key":"27","first-page":"CR417","volume":"17","year":"2011","journal-title":"Medical Science Monitor"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/812503.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/812503.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/812503.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T14:27:35Z","timestamp":1469629655000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/812503\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":27,"alternative-id":["812503","812503"],"URL":"https:\/\/doi.org\/10.1155\/2015\/812503","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}