{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:59:26Z","timestamp":1789430366048,"version":"build-2803163510"},"reference-count":31,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["8151008004000004"],"award-info":[{"award-number":["8151008004000004"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Purposes<\/jats:italic>. To investigate the possible changes in B cell subsets and in B cell expression patterns of lipid rafts (LRs) and F-actin in patients with SLE and whether leflunomide treatment may have effect on these changes.<jats:italic>Methods<\/jats:italic>. The B cell subsets and LRs expression were determined by flow cytometry and confocal microscopy, and F-actin expression was examined by confocal microscopy.<jats:italic>Results<\/jats:italic>. CD27<jats:sup>+<\/jats:sup>IgD<jats:sup>+<\/jats:sup>B cell subsets were significantly decreased while CD38<jats:sup>+<\/jats:sup>CD95<jats:sup>+<\/jats:sup>B cell subsets increased in SLE patients. The LRs levels of B cells were remarkably increased and positively correlated with SLEDAI and anti-dsDNA titer in SLE patients. The expression level of LRs was significantly higher in CD38<jats:sup>+<\/jats:sup>B cells than CD38<jats:sup>\u2212<\/jats:sup>B cells and negatively correlated with C3 levels. The increased expression of LRs was associated with reduced expression of F-actin in the B cells from active SLE patients. Furthermore, in vitro treatment of the cells with A771726 reduced the expression level of LRs, attenuated the overaggregation of LRs, and normalized the distribution of F-actin.<jats:italic>Conclusions<\/jats:italic>. There were abnormalities in B cell subsets and LRs and F-actin expression of B cell from SLE patients. Modulation of B cell expression of LRs and F-actin by LEF could be a potential therapeutic target for SLE.<\/jats:p>","DOI":"10.1155\/2015\/832916","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T21:46:11Z","timestamp":1431985571000},"page":"1-8","source":"Crossref","is-referenced-by-count":14,"title":["Effect of Leflunomide on the Abnormal Expression of Lipid Rafts and F-Actin in B Lymphocytes from Patients with Systemic Lupus Erythematosus"],"prefix":"10.1155","volume":"2015","author":[{"given":"Guang Fu","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 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