{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T11:55:11Z","timestamp":1726919711635},"reference-count":30,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Science and Information Technology of Guangzhou and Hospital Pharmacy Foundation of Guangdong Pharmaceutical Association","award":["2013A25"],"award-info":[{"award-number":["2013A25"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>11Beta-hydroxysteroid dehydrogenase (11<jats:italic>\u03b2<\/jats:italic>-HSD) enzymes control the glucocorticoid (GC) signaling, which is essential in regulating homeostasis. Our previous study revealed that<jats:italic>Eclipta prostrata<\/jats:italic>(EP) affected the activity and expression of 11<jats:italic>\u03b2<\/jats:italic>-HSD enzymes which might improve the efficacy and reduce the adverse drug effects of glucocorticoid in patients undergoing combinational therapy. However, it is still unclear which composition of EP plays a major role and how it works. In this paper, we chose<jats:italic>Luteolin<\/jats:italic>which is one of the main ingredients of EP and evaluated its effect and metabolism in combination with prednisone. The effects of different concentrations of<jats:italic>Luteolin<\/jats:italic>extract on prednisone\/prednisolone metabolism indicated the enzyme activity of 11<jats:italic>\u03b2<\/jats:italic>-HSD, so the production rate (pmol\/min per mg protein) of metabolites was used to indicate enzyme activity. Furthermore, we explored the influence of<jats:italic>Luteolin<\/jats:italic>on gene and protein expressions of 11<jats:italic>\u03b2<\/jats:italic>-HSD I\/II in rat liver and kidney tissue. Our results showed that oral administration of<jats:italic>Luteolin<\/jats:italic>significantly increased the gene and protein expressions of hepatic 11<jats:italic>\u03b2<\/jats:italic>-HSD I and renal 11<jats:italic>\u03b2<\/jats:italic>-HSD II, which may improve the efficacy and reduce the adverse drug effect of glucocorticoid in clinical application. A potential clinical value of<jats:italic>Luteolin<\/jats:italic>would also be indicated in combination therapy with prednisone for the treatment of nephrotic syndrome.<\/jats:p>","DOI":"10.1155\/2015\/834124","type":"journal-article","created":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T20:09:30Z","timestamp":1435262970000},"page":"1-7","source":"Crossref","is-referenced-by-count":5,"title":["Effect of<i>Luteolin<\/i>on 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney"],"prefix":"10.1155","volume":"2015","author":[{"given":"Lei","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, The First Affiliated Hospital, Sun Yat-Sen University, 58 Zhongshan No. 2 Road, Guangzhou, Guangdong 510080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The First Affiliated Hospital, Sun Yat-Sen University, 58 Zhongshan No. 2 Road, 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