{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:54:33Z","timestamp":1791424473117,"version":"4.3.3"},"reference-count":38,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Multiple resistance of<jats:italic>Streptococcus pneumoniae<\/jats:italic>is generally associated with their unique recombination-mediated genetic plasticity and possessing the mobile genetic elements. The aim of our study was to detect antibiotic resistance determinants and conjugative transposons in 138 antibiotic-resistant pneumococcal strains isolated from nasopharynx of healthy young children from Lublin, Poland. These strains resistant to tetracycline and\/or to chloramphenicol\/erythromycin\/clindamycin were tested by PCR using the specific genes as markers. The presence of Tn<jats:italic>916<\/jats:italic>family transposons, carrying<jats:italic>tet<\/jats:italic>(M) and<jats:italic>int\/xis<\/jats:italic>Tn<jats:italic>916<\/jats:italic>, was observed in all of the tested strains. Tn<jats:italic>916<\/jats:italic>was detected in 16 strains resistant only to tetracycline. Tn<jats:italic>6002<\/jats:italic>and Tn<jats:italic>3872<\/jats:italic>-related element were found among 99<jats:italic>erm<\/jats:italic>(B)-carrying strains (83.8% and 3.0%, resp.). Eight strains harbouring<jats:italic>mef<\/jats:italic>(E) and<jats:italic>erm<\/jats:italic>(B) genes were detected, suggesting the presence of Tn<jats:italic>2010<\/jats:italic>and Tn<jats:italic>2017<\/jats:italic>transposons. Among 101 chloramphenicol-resistant strains, two variants of Tn<jats:italic>5252<\/jats:italic>-related transposon were distinguished depending on the presence of<jats:italic>int\/xis5252<\/jats:italic>genes specific for<jats:italic>cat<\/jats:italic>gene-containing Tn<jats:italic>5252<\/jats:italic>(75.2% of strains) or<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi>i<\/mml:mi><mml:mi>n<\/mml:mi><mml:mi>t<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>Sp<\/mml:mtext><mml:mn>23<\/mml:mn><mml:mtext>FST<\/mml:mtext><mml:mn>81<\/mml:mn><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>gene, specific for<jats:italic>cat<\/jats:italic>-containing ICESp23FST81 element (24.8% of strains). In 6 strains Tn<jats:italic>916<\/jats:italic>-like and Tn<jats:italic>5252<\/jats:italic>-like elements formed a Tn<jats:italic>5253<\/jats:italic>-like structure. Besides clonal dissemination of resistant strains of pneumococci in the population, horizontal transfer of conjugative transposons is an important factor of the high prevalence of antibiotic resistance.<\/jats:p>","DOI":"10.1155\/2015\/836496","type":"journal-article","created":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T18:03:34Z","timestamp":1427479414000},"page":"1-6","source":"Crossref","is-referenced-by-count":16,"title":["Resistance Determinants and Their Association with Different Transposons in the Antibiotic-Resistant<i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1155","volume":"2015","author":[{"given":"Izabela","family":"Korona-Glowniak","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radoslaw","family":"Siwiec","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Malm","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s1473-3099(04)00938-7"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1086\/345556"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00283.x"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01232.x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1311-1315.2001"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01484-06"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/bf00333582"},{"issue":"9","key":"8","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1128\/AAC.42.9.2312","volume":"42","year":"1998","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00535"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1089\/107662903322286445"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1086\/321001"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00598-07"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00277-06"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01868-09"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.40.3.774-778.2002"},{"issue":"5","key":"16","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1128\/jb.173.5.1617-1622.1991","volume":"173","year":"1991","journal-title":"Journal of Bacteriology"},{"issue":"3","key":"17","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1128\/jb.166.3.978-984.1986","volume":"166","year":"1986","journal-title":"Journal of Bacteriology"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1097(95)00455-6"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/732901"},{"issue":"11","key":"20","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.1128\/AAC.40.11.2562","volume":"40","year":"1996","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.41.1.428-431.2003"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.393-395.2000"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01457-07"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm120"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1128\/aac.44.3.790-793.2000"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01087-10"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01384-06"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg321"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0321-5"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.43.3.1294-1300.2005"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.3201\/eid1106.050222"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093752"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.43.1.168-173.2005"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-228"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04875.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.10.002"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.03.002"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01551-07"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/836496.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/836496.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/836496.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T13:45:40Z","timestamp":1566481540000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/836496\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":38,"alternative-id":["836496","836496"],"URL":"https:\/\/doi.org\/10.1155\/2015\/836496","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}