{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:48:52Z","timestamp":1791924532561,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2009\/52244-5"],"award-info":[{"award-number":["2009\/52244-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>In breast cancer, lipid metabolic alterations have been recognized as potential oncogenic stimuli that may promote malignancy. To investigate whether the oncogenic nature of lipogenesis closely depends on the overexpression of HER2 protooncogene, the normal breast cell line, HB4a, was transfected with HER2 cDNA to obtain HER2-overexpressing HB4aC5.2 cells. Both cell lines were treated with trastuzumab and docosahexaenoic acid. HER2 overexpression was accompanied by an increase in the expression of lipogenic genes involved in uptake (CD36), transport (FABP4), and storage (DGAT) of exogenous fatty acids (FA), as well as increased activation of \u201cde novo\u201d FA synthesis (FASN). We further investigate whether this lipogenesis reprogramming might be regulated by mTOR\/PPAR<jats:italic>\u03b3<\/jats:italic>pathway. Inhibition of the mTORC1 pathway markers, p70S6\u2009K1, SREBP1, and LIPIN1, as well as an increase in DEPTOR expression (the main inhibitor of the mTOR) was detected in HB4aC5.2. Based on these results, a PPAR<jats:italic>\u03b3<\/jats:italic>selective antagonist, GW9662, was used to treat both cells lines, and the lipogenic genes remained overexpressed in the HB4aC5.2 but not HB4a cells. DHA treatment inhibited all lipogenic genes (except for FABP4) in both cell lines yet only induced death in the HB4aC5.2 cells, mainly when associated with trastuzumab. Neither trastuzumab nor GW9662 alone was able to induce cell death. In conclusion, oncogenic transformation of breast cells by HER2 overexpression may require a reprogramming of lipogenic genetic that is independent of mTORC1 pathway and PPAR<jats:italic>\u03b3<\/jats:italic>activity. This reprogramming was inhibited by DHA.<\/jats:p>","DOI":"10.1155\/2015\/838652","type":"journal-article","created":{"date-parts":[[2015,11,12]],"date-time":"2015-11-12T16:01:31Z","timestamp":1447344091000},"page":"1-13","source":"Crossref","is-referenced-by-count":34,"title":["Docosahexaenoic Acid Modulates a HER2-Associated Lipogenic Phenotype, Induces Apoptosis, and Increases Trastuzumab Action in HER2-Overexpressing Breast Carcinoma Cells"],"prefix":"10.1155","volume":"2015","author":[{"given":"Graziela Rosa","family":"Ravacci","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, School of Medicine, University of S\u00e3o Paulo, LIM 35, Avenida Doutor Arnaldo 455, Cerqueira Cesar, 01246-903 S\u00e3o Paulo, SP, Brazil"},{"name":"Department of Radiology and Oncology, School of Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"},{"name":"Support Group for Research on Food and Nutrition (NAPAN), University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Mitzi","family":"Brentani","sequence":"additional","affiliation":[{"name":"Department of Radiology and Oncology, School of Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"},{"name":"Support Group for Research on Food and Nutrition (NAPAN), University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tharcisio Citr\u00e2ngulo","family":"Tortelli","sequence":"additional","affiliation":[{"name":"Cancer Institute of State of S\u00e3o Paulo (ICESP), S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel Suzana M. 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