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The analysed performance evaluation criteria included the generation of standard curve, reaction efficiency, analytical sensitivity, intra- and interassay repeatability as well as the costs and the practicability of kits, and thermocycling times. We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardless of the machine used, while no difference was detected in reaction efficiency,<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>values, and intra- and interreproducibility between the two methods. The limit of detection at the 95% confidence level varied between 15 to 981\u2009copies\/<jats:italic>\u00b5<\/jats:italic>L and 41 to 171 for one-step kits and two-step kits, respectively. Of the ten kits tested, the most efficient kit was the Quantitect SYBR Green qRT-PCR with a limit of detection at 95% of confidence of 20 and 22\u2009copies\/<jats:italic>\u00b5<\/jats:italic>L on the thermocyclers Rotor gene Q MDx and MX3005P, respectively. The study demonstrated the pivotal influence of the thermocycler on PCR performance for the detection of rabies RNA, as well as that of the master mixes.<\/jats:p>","DOI":"10.1155\/2015\/839518","type":"journal-article","created":{"date-parts":[[2015,2,16]],"date-time":"2015-02-16T21:03:19Z","timestamp":1424120599000},"page":"1-18","source":"Crossref","is-referenced-by-count":19,"title":["Cross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European Bat<i>Lyssavirus<\/i>Type 1"],"prefix":"10.1155","volume":"2015","author":[{"given":"Evelyne","family":"Picard-Meyer","sequence":"first","affiliation":[{"name":"Anses, Laboratory for Rabies and Wildlife, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, European Union Reference Laboratory for Rabies, European Union Reference Laboratory for Rabies Serology, Technop\u00f4le Agricole et V\u00e9t\u00e9rinaire, CS 40009, 54220 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