{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T01:54:27Z","timestamp":1768010067096,"version":"3.49.0"},"reference-count":44,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>.<jats:italic>Bokusoku<\/jats:italic>(BK) is an extract from the<jats:italic>Quercus<\/jats:italic>cortex used in folk medicine for treatment of skin disorders and convergence, and is present in<jats:italic>jumihaidokuto<\/jats:italic>, a traditional Japanese medicine that is prescribed for purulent skin diseases like acne vulgaris. The excess of sebum production induced by androgen is involved in the development of acne. Our aim is to examine whether BK and its constituents inhibit testosterone metabolism and testosterone-induced sebum synthesis.<jats:italic>Methods<\/jats:italic>. Measurements of 5<jats:italic>\u03b1<\/jats:italic>-reductase activity and lipogenesis were performed using rat liver microsomes and hamster sebocytes, respectively.<jats:italic>Results<\/jats:italic>. BK dose-dependently reduced the conversion of testosterone to a more active androgen, dihydrotestosterone in a 5<jats:italic>\u03b1<\/jats:italic>-reductase enzymatic reaction. Twenty polyphenols in BK categorized as gallotannin, ellagitannin, and flavonoid were identified by LC-MS\/MS. Nine polyphenols with gallate group, tetragalloyl glucose, pentagalloyl glucose, eugeniin, 1-desgalloyl eugeniin, casuarinin, castalagin, stenophyllanin C, (\u2212)-epicatechin gallate, and (\u2212)-epigallocatechin gallate, inhibited testosterone metabolism. In particular, pentagalloyl glucose showed the strongest activity. BK and pentagalloyl glucose suppressed testosterone-induced lipogenesis, whereas they weakly inhibited the lipogenic action of insulin.<jats:italic>Conclusions<\/jats:italic>. BK inhibited androgen-related pathogenesis of acne, testosterone conversion, and sebum synthesis, partially through 5<jats:italic>\u03b1<\/jats:italic>-reductase inhibition, and has potential to be a useful agent in the therapeutic strategy of acne.<\/jats:p>","DOI":"10.1155\/2015\/853846","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T21:04:01Z","timestamp":1422565441000},"page":"1-9","source":"Crossref","is-referenced-by-count":42,"title":["Inhibition of Rat 5<i>\u03b1<\/i>-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of<i>Quercus acutissima<\/i>Cortex"],"prefix":"10.1155","volume":"2015","author":[{"given":"Junichi","family":"Koseki","sequence":"first","affiliation":[{"name":"Tsumura Research Laboratories, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, 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