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The nematode resistance for drugs has been reported for livestock, highlighting the importance for development of new anthelmintic compounds. The aim of the current study was to apply and compare fluorimetric techniques using Sytox and propidium iodide for evaluating the viability of<jats:italic>C. elegans<\/jats:italic>larvae after treatment with anthelmintic drugs. These fluorescent markers were efficient to stain larvae treated with ivermectin and albendazole sulfoxide. We observed that densitometric values were proportional to the concentration of dead larvae stained with both markers. Furthermore, data on motility test presented an inverse correlation with fluorimetric data when ivermectin was used. Our results showed that lower concentrations of drugs were effective to interfere in the processes of cellular transport while higher drugs concentrations were necessary in order to result in any damage to cell integrity. The methodology described in this work might be useful for studies that aim to evaluate the viability of nematodes, particularly for testing of new anthelminthic compounds using an easy, economic, reproducible, and no time-consuming technique.<\/jats:p>","DOI":"10.1155\/2015\/879263","type":"journal-article","created":{"date-parts":[[2015,3,20]],"date-time":"2015-03-20T01:20:17Z","timestamp":1426814417000},"page":"1-7","source":"Crossref","is-referenced-by-count":36,"title":["A New Methodology for Evaluation of Nematode Viability"],"prefix":"10.1155","volume":"2015","author":[{"given":"Sebasti\u00e3o Rodrigo","family":"Ferreira","sequence":"first","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Ant\u00f4nio Oliveira","family":"Mendes","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian Lacerda","family":"Bueno","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson Victor","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Universidade Federal de Vi\u00e7osa, Avenida P.H. 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