{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:28:49Z","timestamp":1740169729522,"version":"3.37.3"},"reference-count":19,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["SIVILLA 9502-121","DAIC 46728"],"award-info":[{"award-number":["SIVILLA 9502-121","DAIC 46728"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["SIVILLA 9502-121","DAIC 46728"],"award-info":[{"award-number":["SIVILLA 9502-121","DAIC 46728"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>During amoebic liver abscess (ALA) formation in susceptible animals, immune response is regulated by prostaglandin E<jats:sub>2<\/jats:sub>(PGE<jats:sub>2<\/jats:sub>) dependent mechanisms. The aim of this study was to analyze the effect of misoprostol (MPL), a PGE<jats:sub>1<\/jats:sub>analogue, on ALA formation in BALB\/c mice. Male mice from BALB\/c strain were intrahepatically infected with<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>7.5<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn>5<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>trophozoites of<jats:italic>E. histolytica<\/jats:italic>strain HM1:IMSS and treated with 10<jats:sup>\u22124<\/jats:sup>\u2009M of MPL daily until sacrifice at 2, 4, and 7 days postinfection (p.i.). ALA formation was evaluated at 2, 4, and 7 days postinfection; trophozoite morphology was analyzed using immunohistochemistry and image analysis. Results showed an increase in frequency of ALA formation in infected and MPL-treated mice only at 2 days p.i. (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.03<\/mml:mn><\/mml:math>). A significant diminution in the size of trophozoites was detected in abscesses from mice independently of MPL treatment (from<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn>5.8<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>1.1<\/mml:mn><\/mml:math>\u2009<jats:italic>\u00b5<\/jats:italic>m at 2 days p.i. to<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn>2.7<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>1.9<\/mml:mn><\/mml:math>\u2009<jats:italic>\u00b5<\/jats:italic>m at 7 days p.i.) compared with trophozoites dimensions observed in susceptible hamsters (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mn>9.6<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>2.7<\/mml:mn><\/mml:math>\u2009<jats:italic>\u00b5<\/jats:italic>m) (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>). These results suggest that MPL treatment may modify the adequate control of inflammatory process to allow the persistence of trophozoites in the liver; however, natural resistance mechanisms cannot be discarded.<\/jats:p>","DOI":"10.1155\/2015\/898467","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T21:48:01Z","timestamp":1431985681000},"page":"1-7","source":"Crossref","is-referenced-by-count":1,"title":["Morphological Findings in Trophozoites during Amoebic Abscess Development in Misoprostol-Treated BALB\/c Mice"],"prefix":"10.1155","volume":"2015","author":[{"given":"Andr\u00e9s","family":"Aceves-Cano","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Aut\u00f3noma de Chihuahua, Circuito No. 1, Nuevo Campus Universitario, 31125 Chihuahua, CHIH, 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