{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:36:43Z","timestamp":1790030203565,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T00:00:00Z","timestamp":1429142400000},"content-version":"vor","delay-in-days":105,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Stefania Consenti e Francesca Gelleni","award":["RC41\/08 e RC 01\/09"],"award-info":[{"award-number":["RC41\/08 e RC 01\/09"]}]},{"DOI":"10.13039\/501100004726","name":"Ministry of Health","doi-asserted-by":"publisher","award":["RC41\/08 e RC 01\/09"],"award-info":[{"award-number":["RC41\/08 e RC 01\/09"]}],"id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>To evaluate the efficacy of an association of N\u2010acetyl cystein, alpha\u2010lipoic acid, and bromelain (NAC\/LA\/Br) in the treatment of endometriosis we set up a new <jats:italic>in vivo<\/jats:italic> murine model. We explored the anti\u2010inflammatory and proapoptotic effect of this combination on human endometriotic endothelial cells (EECs) and on endothelial cells isolated from normal uterus (UtMECs). We implanted fragments of human endometriotic cysts intraperitoneally into SCID mice to evaluate the efficacy of NAC\/LA\/Br treatment. UtMECs and EECs, untreated or treated with NAC\/LA\/Br, were activated with the proinflammatory stimulus TNF\u2010<jats:italic>\u03b1<\/jats:italic> and their response in terms of VCAM1 expression was evaluated. The proapoptotic effect of higher doses of NAC\/LA\/Br on UtMECs and EECs was measured with a fluorogenic substrate for activated caspases 3 and 7. The preincubation of EECs with NAC\/LA\/Br prior to cell stimulation with TNF\u2010<jats:italic>\u03b1<\/jats:italic> prevents the upregulation of the expression of the inflammatory \u201cmarker\u201d VCAM1. Furthermore NAC\/LA\/Br were able to induce EEC, but not UtMEC, apoptosis. Finally, the novel mouse model allowed us to demonstrate that mice treated with NAC\/LA\/Br presented a lower number of cysts, smaller in size, compared to untreated mice. Our findings suggest that these dietary supplements may have potential therapeutic uses in the treatment of chronic inflammatory diseases like endometriosis.<\/jats:p>","DOI":"10.1155\/2015\/918089","type":"journal-article","created":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T00:09:47Z","timestamp":1429229387000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["The Combination of N\u2010Acetyl Cysteine, Alpha\u2010Lipoic Acid, and Bromelain Shows High Anti\u2010Inflammatory Properties in Novel <i>In Vivo<\/i> and <i>In Vitro<\/i> Models of Endometriosis"],"prefix":"10.1155","volume":"2015","author":[{"given":"C.","family":"Agostinis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Zorzet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"De Leo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zauli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"De Seta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Bulla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,16]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1333478"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/del339"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-005-0391-x"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2011.650662"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal049"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000181321"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.06.014"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.12.058"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.8.1592209"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.06.029"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmi049"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.10.018"},{"key":"e_1_2_10_13_2","first-page":"179","article-title":"Conservative management of endometriosis","volume":"79","author":"Kistner R. 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