{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T03:47:52Z","timestamp":1765079272687,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>This study evaluated the<jats:italic>in vitro<\/jats:italic>sensitivity of paired<jats:italic>Leishmania braziliensis<\/jats:italic>samples isolated from the same patient before pentavalent antimonial treatment (Sample A) and after treatment failure or cutaneous leishmaniasis reactivation (Sample B) in patients undergoing intralesional administration or injections (5\u2009mgS<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msup><mml:mrow><mml:mtext>b<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>V<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>\/kg\/d) of meglumine antimoniate. Fourteen samples from 7 patients were studied. After 24\u2009h of drug exposure, 50% lethal dose (LD<jats:sub>50<\/jats:sub>) values for promastigotes ranged from 0.37\u2009mg\/mL to 5.86\u2009mg\/mL for samples obtained before treatment (A) and 0.89\u2009mg\/mL to 7.80\u2009mg\/mL for samples obtained after treatment (B). After 48\u2009h, LD<jats:sub>50<\/jats:sub>values ranged from 0.37\u2009mg\/mL to 5.75\u2009mg\/mL and 0.70\u2009mg\/mL to 7.68\u2009mg\/mL for A and B samples, respectively. After 48\u2009h, LD<jats:sub>50<\/jats:sub>values for amastigotes ranged from 11.7 to 44.3\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL for A samples and 13.7 to 52.7\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL for B samples. Of 7 patients, 1 discontinued treatment and 6 were cured after retreatment with amphotericin B (4 cases) or meglumine antimoniate (2 cases). Overall the B samples had higher LD<jats:sub>50<\/jats:sub>values than A samples; however the difference was not significant. These results do not support the hypothesis that low-dose and intralesional treatments induce selection of resistant parasites<jats:italic>in vitro<\/jats:italic>and suggest that other factors may influence therapeutic outcome in patients with poor response to initial treatment.<\/jats:p>","DOI":"10.1155\/2015\/943236","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T16:00:49Z","timestamp":1422720049000},"page":"1-6","source":"Crossref","is-referenced-by-count":11,"title":["<i>In Vitro<\/i>Sensitivity of Paired<i>Leishmania<\/i>(<i>Viannia<\/i>)<i>braziliensis<\/i>Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis"],"prefix":"10.1155","volume":"2015","author":[{"given":"Cibele","family":"Baptista","sequence":"first","affiliation":[{"name":"N\u00facleo de Biosseguran\u00e7a, Bio-Manguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-900 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana de Freitas Campos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-900 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de F\u00e1tima","family":"Madeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-900 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonor Laura Pinto","family":"Leon","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica de Tripanosomat\u00eddeos, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-360 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Concei\u00e7\u00e3o-Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-360 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando de Oliveira","family":"Schubach","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica e Vigil\u00e2ncia em Leishmanioses, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, 21040-900 Rio de Janeiro, RJ, 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