{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:20:14Z","timestamp":1773753614760,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. Clotiapine is an atypical antipsychotic of the dibenzothiazepine class introduced in a few European countries since 1970, efficient in treatment-resistant schizophrenic patients. There is little published data on the therapeutic and toxic concentrations of this drug.<jats:italic>Aims<\/jats:italic>. The aim of the present study is the development and validation of a method that allows the detection and quantification of clotiapine in blood and urine specimens by gas chromatography-mass spectrometry (GC-MS).<jats:italic>Methods<\/jats:italic>. Validation was performed working on spiked postmortem blood and urine samples. Samples were extracted with liquid-liquid extraction (LLE) technique at pH 8.5 with n-hexane\/dichloromethane (85\/15 v\/v) and analysis was followed by GC-MS. Methadone-d9 was used as internal standard.<jats:italic>Results<\/jats:italic>. The limit of detection (LOD) was 1.2 and 1.3\u2009ng\/mL for urine and blood, respectively, while the lower limit of quantification (LLOQ) was 3.9 and 4.3\u2009ng\/mL, respectively. Linearity, precision, selectivity, accuracy, and recovery were also determined. The method was applied to a postmortem case. The blood and urine clotiapine concentrations were 1.32 and 0.49\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, respectively.<jats:italic>Conclusions<\/jats:italic>. A reliable GC-MS method for the detection and quantification of clotiapine in blood and urine samples has been developed and fully validated and then applied to a postmortem case.<\/jats:p>","DOI":"10.1155\/2015\/972480","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T21:06:23Z","timestamp":1436303183000},"page":"1-5","source":"Crossref","is-referenced-by-count":4,"title":["Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case"],"prefix":"10.1155","volume":"2015","author":[{"given":"Giulio","family":"Mannocchi","sequence":"first","affiliation":[{"name":"Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Viale Regina Elena 336, 00161 Rome, 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