{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:12:30Z","timestamp":1777432350205,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T00:00:00Z","timestamp":1430956800000},"content-version":"vor","delay-in-days":126,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>Chronic periodontitis is a multifactorial inflammatory disease that affects supporting structures of the teeth. Although the gingival response is largely described, little is known about the immune changes in the alveolar bone and neighboring tissues that could indicate periodontal disease (PD) activity. Then, in this study we identified the ongoing inflammatory changes and novel biomarkers for periodontitis in the tissues directly affected by the destructive disease in PD patients. Samples were collected by osteotomy in 17 control subjects during extraction of third molars and 18 patients with advanced PD, in which alveoloplasty was necessary after extraction of teeth with previous extensive periodontal damage. Patients presented mononuclear cells infiltration in the connective tissue next to the bone and higher fibrosis area, along with increased accumulation of IL\u201017<jats:sup>+<\/jats:sup> and TRAP<jats:sup>+<\/jats:sup> cells. The levels of TNF\u2010<jats:italic>\u03b1<\/jats:italic> and MMP\u20102 mRNA were also elevated compared to controls and a positive and significant correlation was observed between TNF\u2010<jats:italic>\u03b1<\/jats:italic> and MMP\u20102 mRNA expression, considering all samples evaluated. In conclusion, nongingival tissues neighboring large periodontal pockets present inflammatory markers that could predict ongoing bone resorption and disease spreading. Therefore, we suggested that the detailed evaluation of these regions could be of great importance to the assessment of disease progression.<\/jats:p>","DOI":"10.1155\/2015\/983782","type":"journal-article","created":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T21:24:05Z","timestamp":1431033845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients"],"prefix":"10.1155","volume":"2015","author":[{"given":"Thiago Alvares","family":"da Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Jos\u00e9 Barbosa","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polyanna Miranda","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier Em\u00edlio Lazo","family":"Chica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio Zorzo","family":"Barcelos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max Antonio Alves","family":"Giani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo Pompermaier","family":"Garlet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Santana","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virmondes","family":"Rodrigues J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise Bertulucci Rocha","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Ribeiro de Barros","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,7]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11-s.2033"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.7.1033"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2011.01706.x"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/515178"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510391792"},{"key":"e_1_2_9_6_2","first-page":"ZC56","article-title":"Estimation of levels of salivary mucin, amylase and total protein in gingivitis and chronic periodontitis patients","volume":"8","author":"Kejriwal S.","year":"2014","journal-title":"Journal of Clinical and Diagnostic Research"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01498.x"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(94)90044-2"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100579"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci113133"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.06.002"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.155.12.5483"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.2008.00463.x"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.1998.00171.x"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2004.00545.x"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000070112.80711.3d"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070159"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.060144"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01107.x"},{"key":"e_1_2_9_20_2","volume-title":"A Couting Method for Measuring the Volumes of Tissue Components in Microscopial Sections","author":"Hally A. 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