{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:53:23Z","timestamp":1783738403962,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Plan Project of Guangdong Province","award":["2012B031800084"],"award-info":[{"award-number":["2012B031800084"]}]},{"name":"Science and Technology Plan Project of Guangdong Province","award":["2014A020212624"],"award-info":[{"award-number":["2014A020212624"]}]},{"name":"Science and Technology Plan Project of Guangdong Province","award":["2012B031800084"],"award-info":[{"award-number":["2012B031800084"]}]},{"name":"Science and Technology Plan Project of Guangdong Province","award":["2014A020212624"],"award-info":[{"award-number":["2014A020212624"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. We investigated whether and how diazoxide can attenuate brain injury after cardiopulmonary resuscitation (CPR) by selective opening of mitochondrial ATP-sensitive potassium (mitoKATP) channels.<jats:italic>Methods<\/jats:italic>. Adult male Sprague-Dawley rats with induced cerebral ischemia (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:math>per group) received an intraperitoneal injection of 0.1% dimethyl sulfoxide (1\u2009mL; vehicle group), diazoxide (10\u2009mg\/kg; DZ group), or diazoxide (10\u2009mg\/kg) plus 5-hydroxydecanoate (5\u2009mg\/kg; DZ + 5-HD group) 30\u2009min after CPR. The control group (sham group,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">5<\/mml:mn><\/mml:math>) underwent sham operation, without cardiac arrest. Mitochondrial respiratory control rate (RCR) was determined. Brain cell apoptosis was assessed using TUNEL staining. Expression of Bcl-2, Bax, and protein kinase C epsilon (PKC<jats:italic>\u03b5<\/jats:italic>) in the cerebral cortex was determined by Western blotting and immunohistochemistry.<jats:italic>Results<\/jats:italic>. The neurological deficit scores (NDS) in the vehicle group decreased significantly at 24\u2009h and 48\u2009h after CPR. Diazoxide significantly improved NDS and mitochondrial RCR after CPR at both time points; 5-HD cotreatment abolished these effects. Diazoxide decreased TUNEL-positive cells following CPR, upregulated Bcl-2 and PKC<jats:italic>\u03b5<\/jats:italic>, downregulated Bax, and increased the Bcl-2\/Bax ratio; 5-HD cotreatment reversed these effects.<jats:italic>Conclusions<\/jats:italic>. Diazoxide attenuates postresuscitation brain injury, protects mitochondrial function, inhibits brain cell apoptosis, and activates the PKC pathway by opening mitoKATP channels.<\/jats:p>","DOI":"10.1155\/2016\/1253842","type":"journal-article","created":{"date-parts":[[2016,8,28]],"date-time":"2016-08-28T17:02:57Z","timestamp":1472403777000},"page":"1-6","source":"Crossref","is-referenced-by-count":6,"title":["Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2858-4733","authenticated-orcid":true,"given":"Haidong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7936-4963","authenticated-orcid":true,"given":"Zitong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China"},{"name":"Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, 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