{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:49Z","timestamp":1740169789175,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Major Project for Infectious Diseases","award":["2016ZX10004001-004","2012ZX10004-215"],"award-info":[{"award-number":["2016ZX10004001-004","2012ZX10004-215"]}]},{"name":"National Science and Technology Major Project for Infectious Diseases","award":["2016ZX10004001-004","2012ZX10004-215"],"award-info":[{"award-number":["2016ZX10004001-004","2012ZX10004-215"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>The 41\u2009kD flagellin of<jats:italic>Borrelia burgdorferi (B. burgdorferi)<\/jats:italic>is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease. Here, we chose 89<jats:italic>B. burgdorferi<\/jats:italic>strains in China, amplified the gene encoding the 41\u2009kD flagellin, and compared the sequences. The results showed that genetic diversity presented in the 41\u2009kD flagellin genes of all 89 strains among the four genotypes of<jats:italic>B. burgdorferi<\/jats:italic>, especially in the genotype of<jats:italic>B. garinii<\/jats:italic>. Some specific mutation sites for each genotype of the 41\u2009kD flagellin genes were found, which could be used for genotyping<jats:italic>B. burgdorferi<\/jats:italic>strains in China. Human B-cell epitope analysis showed that thirteen of 15 nonsynonymous mutations occurred in the epitope region of 41\u2009kD flagellin and thirty of 42 B-cell epitopes were altered due to all 13 nonsynonymous mutations in the epitope region, which may affect the function of the antigen. Nonsynonymous mutations and changed human B-cell epitopes exist in 41\u2009kD flagellin of<jats:italic>B. burgdorferi sensu lato<\/jats:italic>strains; these changes should be considered in serodiagnosis of Lyme disease.<\/jats:p>","DOI":"10.1155\/2016\/1327320","type":"journal-article","created":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T23:31:33Z","timestamp":1479252693000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["Polymorphism of 41\u2009kD Flagellin Gene and Its Human B-Cell Epitope in<i>Borrelia burgdorferi<\/i>Strains of China"],"prefix":"10.1155","volume":"2016","author":[{"given":"Huixin","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuexia","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0849-2826","authenticated-orcid":true,"given":"Qin","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3997-0237","authenticated-orcid":true,"given":"Kanglin","family":"Wan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, 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