{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:35Z","timestamp":1740169775325,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postgraduate Research","award":["PV011\/2011B"],"award-info":[{"award-number":["PV011\/2011B"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Although<jats:italic>Eurycoma longifolia<\/jats:italic>has been studied for erectile function, the blood pressure- (BP-) lowering effect has yet to be verified. Hence, this study aims at investigating the BP-lowering properties of the plant with a view to develop an antihypertensive agent that could also preserve erectile function. Ethanolic root extract was partitioned by hexane, dichloromethane (DCM), ethyl acetate, butanol, and water. The DCM fraction, found to be potent in relaxing phenylephrine- (PE-) precontracted rat aortic rings, was further purified by column chromatography. Subfraction DCM-II, being the most active in relaxing aortae, was studied for effects on the renin-angiotensin and kallikrein-kinin systems in aortic rings. The effect of DCM-II on angiotensin-converting enzyme (ACE) activity was also evaluated<jats:italic>in vitro<\/jats:italic>. Results showed that DCM-II reduced (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>) the contractions evoked by angiotensin I and angiotensin II (Ang II). In PE-precontracted rings treated with DCM-II, the Ang II-induced contraction was attenuated (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>) while bradykinin- (BK-) induced relaxation enhanced (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>).<jats:italic>In vitro<\/jats:italic>, DCM-II inhibited (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>) the activity of ACE. These data demonstrate that the vasodilatory effect of DCM-II appears to be mediated<jats:italic>via<\/jats:italic>inhibition of Ang II type 1 receptor and ACE as well as enhancement of Ang II type 2 receptor activation and BK activity.<\/jats:p>","DOI":"10.1155\/2016\/1361508","type":"journal-article","created":{"date-parts":[[2016,10,9]],"date-time":"2016-10-09T17:01:27Z","timestamp":1476032487000},"page":"1-9","source":"Crossref","is-referenced-by-count":2,"title":["First Report of<i>Eurycoma longifolia<\/i>Jack Root Extract Causing Relaxation of Aortic Rings in Rats"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-0746","authenticated-orcid":true,"given":"Bae Huey","family":"Tee","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6138-1271","authenticated-orcid":true,"given":"See Ziau","family":"Hoe","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4700-1572","authenticated-orcid":true,"given":"Swee Hung","family":"Cheah","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-7289","authenticated-orcid":true,"given":"Sau Kuen","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, 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