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Calcium channel blockers (CCBs) are drugs in clinical use for hypertension and other heart pathologies. Based on previous reports about the antileishmanial activity of dihydropyridine\u2010CCBs, this work aimed to investigate whether the <jats:italic>in vitro<\/jats:italic> anti\u2010<jats:italic>Leishmania infantum<\/jats:italic> and anti\u2010<jats:italic>Trypanosoma cruzi<\/jats:italic> activities of this therapeutic class would be shared by other non\u2010dihydropyridine\u2010CCBs. Except for amrinone, our results demonstrated antiprotozoal activity for fendiline, mibefradil, and lidoflazine, with IC<jats:sub>50<\/jats:sub> values in a range between 2 and 16\u2009<jats:italic>\u03bc<\/jats:italic>M and Selectivity Index between 4 and 10. Fendiline demonstrated depolarization of mitochondrial membrane potential, with increased reactive oxygen species production in amlodipine and fendiline treated <jats:italic>Leishmania<\/jats:italic>, but without plasma membrane disruption. Finally, <jats:italic>in vitro<\/jats:italic> combinations of amphotericin B, miltefosine, and pentamidine against <jats:italic>L. infantum<\/jats:italic> showed in isobolograms an additive interaction when these drugs were combined with fendiline, resulting in overall mean sum of fractional inhibitory concentrations between 0.99 and 1.10. These data demonstrated that non\u2010dihydropyridine\u2010CCBs present antiprotozoal activity and could be useful candidates for future <jats:italic>in vivo<\/jats:italic> efficacy studies against Leishmaniasis and Chagas\u2019 disease.<\/jats:p>","DOI":"10.1155\/2016\/1523691","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T21:01:08Z","timestamp":1454014868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Investigation of Calcium Channel Blockers as Antiprotozoal Agents and Their Interference in the Metabolism of <i>Leishmania (L.) infantum<\/i>"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4418-0838","authenticated-orcid":false,"given":"Juliana 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