{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T07:37:05Z","timestamp":1759563425088,"version":"3.37.3"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technology and Innovation Malaysia","award":["MOSTI 09-05-IFN-BPH-001","FS344\/2008C"],"award-info":[{"award-number":["MOSTI 09-05-IFN-BPH-001","FS344\/2008C"]}]},{"name":"Fundamental Research Grant (FRGS-MOHE)","award":["MOSTI 09-05-IFN-BPH-001","FS344\/2008C"],"award-info":[{"award-number":["MOSTI 09-05-IFN-BPH-001","FS344\/2008C"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Plasmodium falciparum<\/jats:italic>mitogen-activated protein (MAP) kinases, a family of enzymes central to signal transduction processes including inflammatory responses, are a promising target for antimalarial drug development. Our study shows for the first time that the<jats:italic>P. falciparum<\/jats:italic>specific MAP kinase 2 (PfMAP2) is colocalized in the nucleus of all of the asexual erythrocytic stages of<jats:italic>P. falciparum<\/jats:italic>and is particularly elevated in its phosphorylated form. It was also discovered that PfMAP2 is expressed in its highest quantity during the early trophozoite (ring form) stage and significantly reduced in the mature trophozoite and schizont stages. Although the phosphorylated form of the kinase is always more prevalent, its ratio relative to the nonphosphorylated form remained constant irrespective of the parasites\u2019 developmental stage. We have also shown that the TSH motif specifically renders PfMAP2 genetically divergent from the other plasmodial MAP kinase activation sites using Neighbour Joining analysis. Furthermore, TSH motif-specific designed antibody is crucial in determining the location of the expression of the PfMAP2 protein. However, by using immunoelectron microscopy, PPfMAP2 were detected ubiquitously in the parasitized erythrocytes. In summary, PfMAP2 may play a far more important role than previously thought and is a worthy candidate for research as an antimalarial.<\/jats:p>","DOI":"10.1155\/2016\/1645097","type":"journal-article","created":{"date-parts":[[2016,7,25]],"date-time":"2016-07-25T17:00:56Z","timestamp":1469466056000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["Phosphorylated and Nonphosphorylated PfMAP2 Are Localized in the Nucleus, Dependent on the Stage of<i>Plasmodium falciparum<\/i>Asexual Maturation"],"prefix":"10.1155","volume":"2016","author":[{"given":"Farah Aida","family":"Dahalan","sequence":"first","affiliation":[{"name":"School of Biosciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasidah Mohd","family":"Sidek","sequence":"additional","affiliation":[{"name":"School of Biosciences & Biotechnology, Faculty of Science and 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