{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T15:30:18Z","timestamp":1767108618423,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in<jats:italic>E. coli<\/jats:italic>obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as<jats:italic>E. coli<\/jats:italic>and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in<jats:italic>E. coli<\/jats:italic>. PCR screening showed the presence of four genes, namely,<jats:italic>rrs<\/jats:italic>,<jats:italic>aacC2<\/jats:italic>,<jats:italic>aacA-aphD<\/jats:italic>, and<jats:italic>aphA3<\/jats:italic>, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in<jats:italic>E. coli<\/jats:italic>from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients.<\/jats:p>","DOI":"10.1155\/2016\/1875865","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:01:28Z","timestamp":1466010088000},"page":"1-5","source":"Crossref","is-referenced-by-count":11,"title":["Identification of Genes Coding Aminoglycoside Modifying Enzymes in<i>E. coli<\/i>of UTI Patients in India"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-4419","authenticated-orcid":true,"given":"Abdul Rouf","family":"Mir","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasir","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdous Ahmad","family":"Dar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sekhar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, The New College, Chennai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"doi-asserted-by":"publisher","key":"1","DOI":"10.1038\/nrurol.2015.199"},{"doi-asserted-by":"publisher","key":"2","DOI":"10.1038\/nrmicro3432"},{"doi-asserted-by":"publisher","key":"3","DOI":"10.1378\/chest.14-2171"},{"doi-asserted-by":"publisher","key":"4","DOI":"10.15171\/bi.2015.15"},{"doi-asserted-by":"publisher","key":"5","DOI":"10.4103\/0253-7613.161258"},{"issue":"4","key":"6","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1128\/AAC.43.4.727","volume":"43","year":"1999","journal-title":"Antimicrobial Agents and Chemotherapy"},{"doi-asserted-by":"publisher","key":"7","DOI":"10.1371\/journal.pone.0017224"},{"doi-asserted-by":"publisher","key":"8","DOI":"10.1016\/j.drup.2010.08.003"},{"doi-asserted-by":"publisher","key":"9","DOI":"10.3389\/fcimb.2013.00022"},{"doi-asserted-by":"publisher","key":"10","DOI":"10.1128\/CMR.16.3.430-450.2003"},{"doi-asserted-by":"publisher","key":"11","DOI":"10.1155\/2014\/329157"},{"doi-asserted-by":"publisher","key":"12","DOI":"10.1371\/journal.pone.0049433"},{"doi-asserted-by":"publisher","key":"13","DOI":"10.1023\/a:1016601629518"},{"year":"2006","key":"14"},{"volume-title":"Susceptibility test methods: dilution and disk diffusion method","year":"2003","first-page":"1108","key":"15"},{"doi-asserted-by":"publisher","key":"17","DOI":"10.3389\/fmicb.2014.00463"},{"doi-asserted-by":"publisher","key":"18","DOI":"10.1016\/j.mjafi.2014.08.007"},{"doi-asserted-by":"publisher","key":"19","DOI":"10.1097\/mph.0000000000000420"},{"doi-asserted-by":"publisher","key":"20","DOI":"10.7860\/jcdr\/2015\/13856.6378"},{"key":"21","first-page":"77","volume":"119","year":"2004","journal-title":"Indian Journal of Medical Research"},{"doi-asserted-by":"publisher","key":"22","DOI":"10.1155\/2016\/8262561"},{"doi-asserted-by":"publisher","key":"23","DOI":"10.5829\/idosi.gjp.2014.8.1.8270"},{"key":"24","first-page":"85","volume":"17","year":"1999","journal-title":"Indian Journal of Medical Microbiology"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2016\/1875865.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2016\/1875865.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2016\/1875865.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T16:04:09Z","timestamp":1568045049000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/tswj\/2016\/1875865\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":23,"alternative-id":["1875865","1875865"],"URL":"https:\/\/doi.org\/10.1155\/2016\/1875865","relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"type":"print","value":"2356-6140"},{"type":"electronic","value":"1537-744X"}],"subject":[],"published":{"date-parts":[[2016]]}}}