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Considering that presynaptic toxins interfere into the machinery involved in neurotransmitter release (synaptophysin, synaptobrevin, and SNAP25 proteins), the main objective of this communication is to analyze, by immunofluorescence and western blotting, the expression of the synaptic proteins, synaptophysin, synaptobrevin, and SNAP25 and by myography, light, and transmission electron microscopy the pathology of motor nerve terminals and skeletal muscle fibres of chick biventer cervicis preparations (CBC) exposed<jats:italic>in vitro<\/jats:italic>to BmjeTX-I and BmjeTX-II toxins from<jats:italic>B. marajoensis<\/jats:italic>venom. CBC incubated with toxins showed irreversible twitch tension blockade and unaffected KCl- and ACh-evoked contractures, and the positive colabelling of acetylcholine receptors confirmed that their action was primarily at the motor nerve terminal. Hypercontraction and loose myofilaments and synaptic vesicle depletion and motor nerve damage indicated that the toxins displayed both myotoxic and neurotoxic effect. The blockade resulted from interference on synaptophysin, synaptobrevin, and SNAP25 proteins leading to the conclusion that BmjeTX-I and BmjeTX-II affected neurotransmitter release machinery by preventing the docking of synaptic vesicles to the axolemma of the nerve terminal.<\/jats:p>","DOI":"10.1155\/2016\/2053459","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T06:05:37Z","timestamp":1471586737000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from<i>Bothrops marajoensis<\/i>(Maraj\u00f3 Lancehead) Snake Venom"],"prefix":"10.1155","volume":"2016","author":[{"given":"Antonio","family":"Lisboa","sequence":"first","affiliation":[{"name":"Multidisciplinary Research Laboratory, S\u00e3o Francisco University (USF), Avenida S\u00e3o Francisco de Assis 218, Jardim S\u00e3o Jos\u00e9, 12916-350 Bragan\u00e7a Paulista, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Melar\u00e9","sequence":"additional","affiliation":[{"name":"Multidisciplinary Research Laboratory, S\u00e3o Francisco University (USF), Avenida S\u00e3o Francisco de Assis 218, Jardim S\u00e3o Jos\u00e9, 12916-350 Bragan\u00e7a Paulista, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junia R. 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